Non-transparent Scaffold Imaging
Non-transparent Scaffold Imaging - Related Videos
07:28
Imaging Cell Viability on Non-transparent Scaffolds — Using the Example of a Novel Knitted Titanium Implant
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Cited by 6 •
2016
Here we present a fluorophore based imaging technique to detect cell viability on a non-transparent titanium scaffold as well as to detect glimpses of the scaffold impurities. This protocol troubleshoots the drawback of imaging cell-cell or cell-metal interactions on non-transparent scaffolds.
14:49
Self-reporting Scaffolds for 3-Dimensional Cell Culture
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Cited by 7 •
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Biocompatible pH responsive sol-gel nanosensors can be incorporated into poly(lactic-co-glycolic acid) (PLGA) electrospun scaffolds. The produced self-reporting scaffolds can be used for in situ monitoring of microenvironmental conditions whilst culturing cells upon the scaffold. This is beneficial as the 3D cellular construct can be monitored in real-time without disturbing the experiment.
09:32
Electrospun Nanofiber Scaffolds with Gradations in Fiber Organization
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Cited by 5 •
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Here, we present a protocol to fabricate electrospun nanofiber scaffolds with gradated organization of fibers and explore their applications in regulating cell morphology/orientation. Gradients with regard to physical and chemical properties of the nanofiber scaffolds offer a wide variety of applications in the biomedical field.
04:33
Decellularized Porcine Esophageal Scaffold Preparation: A Technique to Generate Acellular Scaffold from a Pig Esophagus
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2023
In this video, we demonstrate the preparation of a decellularized pig esophagus scaffold, with its biochemical architecture and composition preserved, for tissue engineering applications.
08:35
Elastomeric PGS Scaffolds in Arterial Tissue Engineering
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Cited by 21 •
2011
Elastomeric PGS scaffolds with vascular smooth muscle cells cultured in a pulsatile flow bioreactor may lead to promising small-diameter arterial constructs with native ECM production in a relatively short culture period.
FAQs
A surface view cannot reveal how pores, cells, and biomaterials are arranged throughout a scaffold that blocks or scatters light. Measurements from multiple depths provide access to internal regions and support reconstruction of their spatial distribution. This deeper perspective is important when evaluating whether the construct has an architecture and cellular organization compatible with tissue formation.
The imaging approach acquires signals at different depths and uses those measurements to separate scaffold features from biological components. This distinction allows investigators to examine biomaterial structure alongside cell attachment and remodeling instead of treating the construct as a single undifferentiated volume. The resulting spatial information strengthens assessment of how the scaffold and developing tissue interact.
Strong light blocking or scattering limits visibility at the scaffold surface and makes internal features difficult to observe directly. Non-transparent scaffold imaging addresses this limitation by gathering information through the construct rather than depending on a single exposed plane. Recognizing this optical constraint helps researchers interpret internal architecture and biological distribution more reliably.
A basic workflow begins by acquiring signals from multiple depths within the construct. The measurements are then used to distinguish scaffold features from biological components and reconstruct their spatial distribution. Investigators can examine the resulting representation for pore organization, cell attachment, and remodeling. These observations provide evidence for how the scaffold is functioning during tissue development.
It is useful when researchers need to evaluate internal architecture rather than judge a scaffold from its exterior alone. Imaging can reveal pore organization and the distribution of biomaterials within the construct, supporting scaffold design decisions and manufacturing quality control. The same information can help identify whether a fabricated structure has the internal organization expected for tissue-engineering use.
The measurements can document pore organization, cell attachment, and scaffold remodeling within an engineered construct. Together, these outcomes show how biological components are distributed relative to the biomaterial and whether the scaffold is changing as tissue develops. In bioengineering studies, that evidence helps assess whether a construct supports tissue formation and informs interpretation of its performance.