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Chapters
- 00:05Title
- 01:36Preparation and Analysis of pH Responsive Sol-gel Nanosensors
- 04:26Preparation and Analysis of PLGA Scaffolds
- 06:57Nanosensor Location within Mammalian Cells
- 08:36Nanosensor Delivery to Cells Cultured upon Regular PLGA Scaffolds
- 10:33Nanosensor Location within Mammalian Cells
- 11:57Results: Incorporation of Nanosensors into Scaffolds and Cells
- 14:20Conclusion
Biocompatible pH responsive sol-gel nanosensors can be incorporated into poly(lactic-co-glycolic acid) (PLGA) electrospun scaffolds. The produced self-reporting scaffolds can be used for in situ monitoring of microenvironmental conditions whilst culturing cells upon the scaffold. This is beneficial as the 3D cellular construct can be monitored in real-time without disturbing the experiment.
