Particle Characterization
Particle Characterization - Related Videos
11:59
In Situ Characterization of Boehmite Particles in Water Using Liquid SEM
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Cited by 5 •
2017
We present a procedure for real-time imaging and elemental composition analysis of boehmite particles in deionized water by in situ liquid Scanning Electron Microscopy.
06:54
Characterization of Nanocrystal Size Distribution using Raman Spectroscopy with a Multi-particle Phonon Confinement Model
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Cited by 10 •
2015
We demonstrate how to determine the size distribution of semiconductor nanocrystals in a quantitative manner using Raman spectroscopy employing an analytically defined multi-particle phonon confinement model. Results obtained are in excellent agreement with the other size analysis techniques like transmission electron microscopy and photoluminescence spectroscopy.
03:37
Subatomic Particles
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2020
Dalton was only partially correct about the particles that make up matter. All matter is composed of atoms, and atoms are composed of three smaller subatomic particles: protons, neutrons, and electrons. These three particles account for the mass and the charge of an atom. The Discovery of the Electron The first clue about the subatomic structure came at the end of the 19th century when J.J. Thomson discovered the electron using a cathode ray tube. This apparatus consisted of a sealed glass...
13:30
Mass-Sensitive Particle Tracking to Characterize Membrane-Associated Macromolecule Dynamics
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Cited by 3 •
2022
This protocol describes an iSCAT-based image processing and single-particle tracking approach that enables the simultaneous investigation of the molecular mass and the diffusive behavior of macromolecules interacting with lipid membranes. Step-by-step instructions for sample preparation, mass-to-contrast conversion, movie acquisition, and post-processing are provided alongside directions to prevent potential pitfalls.
06:48
Synthesis and Characterization of Self-Assembled Metal-Organic Framework Monolayers Using Polymer-Coated Particles
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2024
A protocol for the synthesis and characterization of self-assembled metal-organic framework monolayers is provided using polymer-grafted, metal-organic framework (MOF) crystals. The procedure shows that polymer-grafted MOF particles can be self-assembled at an air-water interface resulting in well-formed, free-standing, monolayer structures as evidenced by scanning electron microscopy imaging.
FAQs
Dynamic light scattering analyzes fluctuations in scattered light to estimate particle size and size distribution. Because the measurement depends on light-scattering behavior from the population, the resulting data describe the sample collectively rather than documenting visual features particle by particle. This makes it useful for assessing formulated nanoparticles and monitoring whether a preparation remains consistent.
Electrophoretic mobility analysis measures how particles move in an applied electric field, providing information about surface charge. That interfacial property can be evaluated alongside size and composition because it contributes to particle stability, transport, cellular uptake, and biological activity. In bioengineering, the measurement helps connect a particle’s surface characteristics with its intended biological performance.
Microscopy reveals visual features directly, allowing characterization to include particle shape in addition to size-related information. This distinguishes it from approaches that infer population properties from light scattering or particle movement. For bioengineered materials, visual assessment can add structural context when researchers examine nanoparticles, biomaterials, or cell-derived vesicles.
These properties describe different aspects of the same particle population, so measuring them together provides a more complete basis for interpretation. Size and size distribution describe population dimensions, microscopy contributes visual and shape information, composition identifies particle constituents, and surface charge adds interfacial information. Combining these results supports assessment of engineered particles intended for biological use.
A practical plan begins by selecting the properties most relevant to the particle’s intended use, such as size, size distribution, shape, composition, or surface charge. Researchers then match those properties to methods including dynamic light scattering, microscopy, or electrophoretic mobility analysis. This targeted approach supports formulation development, quality control, and reproducibility.
In drug-delivery systems, characterization helps determine whether particle attributes support desired transport, cellular uptake, and biological activity. Measurements can also guide formulation development and quality control by revealing whether preparations have consistent physical and interfacial properties. The same logic applies to nanoparticles, biomaterials, and cell-derived vesicles developed for diagnostic or therapeutic purposes.
In tissue engineering, particle properties can inform how a material is designed and evaluated, particularly when size, shape, composition, or surface charge are relevant to the engineered system. Characterization provides measurements that support reproducibility and comparison among material formulations, helping researchers relate particle features to diagnostic, therapeutic, or tissue-engineering goals.