Tomographic Imaging
Tomographic Imaging - Related Videos
08:41
Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution
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Cited by 11 •
2012
Lensfree optical tomography is a three-dimensional microscopy technique that offers a spatial resolution of <1 μm × <1 μm × <3 μm in x, y and z dimensions, respectively, over a large imaging-volume of 15-100 mm3, which can be particularly useful for integration with lab-on-a-chip platforms.
07:57
Scaled Anatomical Model Creation of Biomedical Tomographic Imaging Data and Associated Labels for Subsequent Sub-surface Laser Engraving (SSLE) of Glass Crystals
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2017
A methodology is described herein for representing anatomical imaging data within crystals. We create scaled three-dimensional models of biomedical imaging data for use in Sub-Surface Laser Engraving (SSLE) of crystal glass. This tool offers a useful complement to computational display or three-dimensionally printed models used within clinical or educational settings.
11:06
3D Printing of Preclinical X-ray Computed Tomographic Data Sets
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Cited by 16 •
2013
Using modern plastic extrusion and printing technologies, it is now possible to quickly and inexpensively produce physical models of X-ray CT data taken in a laboratory. The three -dimensional printing of tomographic data is a powerful visualization, research, and educational tool that may now be accessed by the preclinical imaging community.
12:54
Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo
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Cited by 5 •
2021
This protocol presents a collection of sarcomere, calcium, and macroscopic geometric data from an actively contracting cardiac trabecula ex vivo. These simultaneous measurements are made possible by the integration of three imaging modalities.
05:32
Retrospective Cardiac Gating with A Prototype Small-Animal X-ray Computed Tomograph
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Cited by 2 •
2025
We provide a comprehensive description of the intrinsic retrospective cardiac gating method of the CrumpCAT, a prototype small-animal X-ray computed tomography (CT) scanner designed and constructed at our research institution.
FAQs
Each projection records how the specimen modifies the measurement along a particular viewing direction. When projections are collected around or through the specimen, reconstruction algorithms combine their differing views to estimate internal structure at many locations. The resulting slices are therefore computationally derived from the full set of measurements, rather than copied from a single physical plane.
In computed tomography, x-ray attenuation supplies the contrast used for reconstruction. Regions that attenuate x-rays differently contribute different values to the measured projections, allowing the algorithm to distinguish internal features within reconstructed slices. This makes attenuation contrast useful for examining geometry and structural differences in tissues, biomaterials, and engineered constructs without cutting them open.
Multiple viewing angles are essential because one projection compresses information from structures along its path. Changing the angle supplies complementary measurements, reducing ambiguity about where internal features lie. In tomographic imaging, combining these projections enables a cross-sectional or three-dimensional representation, which is more informative for evaluating internal geometry than any single through-specimen measurement.
Unlike physical sectioning, tomographic imaging preserves the specimen during data collection. That distinction matters when the same tissue, biomaterial, or engineered construct must be assessed more than once or compared across growth or repair. Non-destructive visualization can reveal internal geometry and defects while leaving the sample available for subsequent observation or continued study.
A basic computed tomography workflow begins by positioning the specimen between an x-ray source and detector, acquiring projections at multiple angles, and applying computational reconstruction to those measurements. The output is a set of slices that can be interpreted individually or combined into a three-dimensional representation. Each stage links the measured signals to the internal structure being evaluated.
In bioengineering, tomographic imaging can evaluate tissues, biomaterials, and engineered constructs without physical sectioning. The reconstructed views provide information about geometry, internal structure, and defects, helping investigators examine scaffolds for design-related features or assess devices and constructs. Its value lies in observing internal characteristics within the intact specimen rather than relying only on external inspection.