Interstitial Fluid Sampling
Interstitial Fluid Sampling - Related Videos
03:34
Spatial Measurement of Tumor Interstitial Fluid Pressure: A Method to Measure the Interstitial Fluid Pressure
0 Views •
2023
This video describes a technique of measuring the interstitial fluid pressure within a tumor by perfusion imaging using an image-guided robotic system. Measurements are compared to the intra-tumoral accumulation of liposomes, within a solid tumor.
02:29
Microdialysis of Interstitial Fluid from a Mouse Brain to Isolate Extracellular Proteins
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2025
The video demonstrates the process of using a microdialysis probe to collect extracellular proteins from the interstitial fluid (ISF) of a mouse brain. A microdialysis probe, equipped with a high-molecular-weight cut-off semi-permeable membrane, is inserted into the brain of an anesthetized mouse via an implanted guide cannula. The probe, connected to pumps, allows a physiological buffer to perfuse the brain tissue and mix with the ISF. Proteins in the ISF diffuse across the semi-permeable...
08:50
Use of Cerebral Open-Flow Microperfusion for the Longitudinal Collection of Interstitial Fluid in an Animal Model of Glioblastoma
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2025
In vivo, intercellular communications underpinning many central nervous system diseases, such as glioblastoma (GBM), are notoriously difficult to measure and characterize. Here, we describe procedures of cerebral open-flow microperfusion (cOFM) that can be employed to sample interstitial fluid components in a longitudinal animal model of GBM.
07:41
Three-dimensional Cell Culture Model for Measuring the Effects of Interstitial Fluid Flow on Tumor Cell Invasion
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Cited by 13 •
2012
Interstitial fluid flow is elevated in solid tumors and can modulate tumor cell invasion. Here we describe a technique to apply interstitial fluid flow to cells embedded in a matrix and then measure its effects on cell invasion. This technique can be easily adapted to study other systems.
09:00
Spatial Measurements of Perfusion, Interstitial Fluid Pressure and Liposomes Accumulation in Solid Tumors
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Cited by 6 •
2016
The heterogeneous intra-tumoral accumulation of liposomes has been linked to an abnormal tumor microenvironment. Herein methods are presented to measure tumor microcirculation by perfusion imaging and elevated interstitial fluid pressure (IFP) using an image-guided robotic system. Measurements are compared to the intra-tumoral accumulation of liposomes, determined using volumetric micro-CT imaging.
FAQs
The sampling interface determines which molecules can be recovered and how well the collected fluid represents local tissue chemistry. In microdialysis, a membrane-based probe provides the contact surface for recovery, whereas microneedle-based designs use small needle structures to access tissue. These choices directly affect selectivity, recovery, and tissue disruption.
Minimizing tissue disruption helps preserve the local environment being measured and supports compatibility with repeated or continuous monitoring. Small probes and microneedle-based approaches are valuable because they can access interstitial fluid while limiting disturbance to surrounding tissue. This feature is especially relevant when bioengineers design wearable systems or investigate how tissue responds to therapy.
Three linked factors are especially important: selectivity, molecular recovery, and compatibility with the sensing system. Selectivity influences which compounds are represented, recovery affects the amount available for analysis, and sensor compatibility determines whether the collected fluid can be measured effectively. Improving all three can support more precise physiological measurements.
Interstitial fluid measurements provide information about the chemistry surrounding cells, while blood-based measurements describe a different physiological compartment. Comparing the two can help distinguish local tissue conditions from broader circulating signals. This distinction is useful for examining tissue responses, tracking local biomarkers, and developing measurements that may reveal changes not fully represented in blood.
A typical workflow uses a microdialysis probe or microneedle-based device to access the tissue compartment, recover fluid through the sampling interface, and transfer the collected material for analysis or sensing. The resulting measurement can then be interpreted as information about local glucose, metabolites, drugs, or biomarkers, depending on the experimental goal.
The collected fluid can be analyzed for glucose, metabolites, drugs, and biomarkers. Measuring these compounds helps researchers examine local tissue chemistry rather than relying only on circulating concentrations. The resulting data may support continuous monitoring, evaluation of tissue responses to therapies, or assessment of molecular changes relevant to disease research.
Bioengineering applications include continuous physiological monitoring, wearable biosensor development, disease research, and studies evaluating tissue responses to therapies. The approach is also relevant when engineers seek to connect a sampling interface with a sensor for more immediate measurements. Its value comes from combining access to local chemistry with minimally disruptive device designs.
Future systems can benefit from better sampling selectivity, higher molecular recovery, and stronger compatibility between the sampling interface and sensor. These improvements could make measurements more precise and support real-time physiological monitoring. In bioengineering, integrating these features may help wearable devices capture local tissue changes with greater practical value for research and therapy evaluation.