Transillumination Imaging
Transillumination Imaging - Related Videos
09:59
Transillumination-Assisted Dissection of Specific Stages of the Mouse Seminiferous Epithelial Cycle for Downstream Immunostaining Analyses
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Cited by 18 •
2020
This protocol describes transillumination-assisted microdissection of segments of adult mouse seminiferous tubules representing specific stages of seminiferous epithelial cycle, and cell types therein, and subsequent immunostaining of squash preparations and intact tubule segments.
03:09
Fluorescence Molecular Tomography: An Imaging Technique for In Vivo Imaging of Fluorescent Protein-tagged Glioblastoma Xenografts in Mouse Model
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2025
In this video, we describe fluorescence molecular tomography or FMT for in vivo imaging of fluorescent protein-tagged glioblastoma xenografts in mouse models. The technique is useful for elucidating the effects of anti-tumor therapeutics at the molecular level.
11:51
Mesoscopic Fluorescence Tomography for In-vivo Imaging of Developing Drosophila
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Cited by 9 •
2009
Mesoscopic fluorescence tomography operates beyond the penetration limits of tissue-sectioning fluorescence microscopy. The technique is based on multi-projection illumination and a photon transport description. We demonstrate in-vivo whole-body 3D visualization of the morphogenesis of GFP-expressing wing imaginal discs in Drosophila melanogaster.
05:26
Reporter Enzyme Fluorescence Imaging for In Vivo Tracking of Bacterial Infection
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2025
This video demonstrates the reporter enzyme fluorescence (REF) imaging for in vivo detection of Mycobacterium tuberculosis infection. The infected mice are intraperitoneally injected with a substrate for REF imaging. Upon being hydrolyzed by bacterial β-lactamase enzyme, the substrate emits fluorescence, the detection of which aids evaluation of infection.
13:45
Hybrid µCT-FMT imaging and image analysis
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Cited by 34 •
2015
We describe a protocol for hybrid imaging, combining fluorescence-mediated tomography (FMT) with micro computed tomography (µCT). After fusion and reconstruction, we perform interactive organ segmentation to extract quantitative measurements of the fluorescence distribution.
FAQs
Contrast arises because different parts of a specimen do not transmit light in the same way. Absorption reduces the intensity of particular light paths, while scattering changes how light is redirected and how its spatial distribution reaches the detector. Variations in composition, thickness, and density therefore appear as differences in recorded intensity, allowing internal features to be distinguished without cutting or staining.
The camera or sensor converts transmitted-light differences into a spatially organized image. It records how intensity varies across the specimen, preserving patterns produced by internal differences in optical behavior. This conversion is essential because the useful information is not simply whether light passes through, but how its intensity and distribution change from one location to another.
Transillumination Imaging offers a noninvasive, label-free view that can be obtained with a comparatively simple optical arrangement. Microscopy can therefore be used alongside it rather than being replaced by it. The technique is especially useful when researchers need rapid visualization of internal structure without cutting or staining, while microscopy supplies a complementary imaging approach.
First, position the specimen between an illumination source and the recording device. Then direct light through the specimen and collect the transmitted pattern with a camera or sensor. The recorded intensity and spatial distribution are converted into image contrast, which can be examined for differences related to composition, thickness, density, or internal organization.
Applications described for this approach include soft tissues, microvascular features, embryos, and engineered constructs. These specimens can benefit from internal visualization without sectioning or staining. The method is particularly relevant when rapid, label-free assessment is needed to inspect structure or follow changes during development and fabrication.
In bioengineering, the technique supports assessment of both naturally occurring biological structures and engineered materials or constructs. Researchers can use transmitted-light contrast to visualize internal organization during development or fabrication, including features in embryos, soft tissues, and engineered constructs. Its noninvasive and rapid character makes it useful when repeated structural assessment is desirable.