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Mesoscopic Fluorescence Tomography for In-vivo Imaging of Developing Drosophila
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Mesoscopic Fluorescence Tomography for In-vivo Imaging of Developing Drosophila

Mesoscopic Fluorescence Tomography for In-vivo Imaging of Developing Drosophila

DOI:
10.3791/1510-v

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11:51 min

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August 20, 2009

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, , , , ,
1Center for Systems Biology,Massachusetts General Hospital, 2Institute for Biological and Medical Imaging (IBMI),Technical University of Munich and Helmholtz Center Munich, 3Department of Genetics,Harvard Medical School and Howard Hughes Medical Institute

Chapters

  • 00:05Title
  • 00:54Introduction
  • 01:34Preparation of Drosophila
  • 04:13Mounting Drosophila Pupae for Imaging
  • 05:16Scanning a Tissue of Interest
  • 06:18Time-lapse Imaging
  • 07:58Three Dimensional Reconstruction of Fluorescence
  • 09:59Representative Results/ Outcome
  • 10:58Conclusion

Summary

Automatic Translation

Automatic Translation

Mesoscopic fluorescence tomography operates beyond the penetration limits of tissue-sectioning fluorescence microscopy. The technique is based on multi-projection illumination and a photon transport description. We demonstrate in-vivo whole-body 3D visualization of the morphogenesis of GFP-expressing wing imaginal discs in Drosophila melanogaster.

Tags

Mesoscopic Fluorescence TomographyIn-vivo ImagingDeveloping DrosophilaOptical InterrogationMolecular ChangesFunctional ChangesOptical Imaging MethodsPenetration LimitsOptical MicroscopyDiffusion-imposed LimitsOptical MacroscopyModel OrganismsInsectsAnimal EmbryosSmall Animal ExtremitiesNanometer-resolution Optical ImagingImaging Penetration DepthPhoton ScatteringEntire Embryos ImagingChemical TreatmentScattering ClearancePost-mortem ImagingTwo-photon MicroscopyConfocal MicroscopyDevelopmental BiologyDrug Discovery

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