Adult Topical Assay
Adult Topical Assay - Related Videos
04:43
Topical Testing Assay: A Technique to Evaluate the Insecticidal Potential of Dopamine Receptor Antagonists on Adult Female Mosquitoes by Topical Application
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2025
In this video, we demonstrate the topical testing assay to determine the toxicity of a potential insecticide on adult female mosquitoes. Using a dopamine receptor antagonist that causes lethal inhibition of neurotransmission in mosquitoes, this method describes a way to evaluate the toxic effects of insecticides upon topical application.
09:38
An Assay for Lateral Line Regeneration in Adult Zebrafish
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Cited by 11 •
2014
Because many zebrafish models of neurological and non-neurological diseases are studied in the adult fish rather than the embryo/larvae, we developed a quantitative lateral line regenerative assay that can be applied to adult zebrafish disease models. The assay involved resolution at the 1) neuromast and 2) individual hair cell levels.
04:31
Taste Preference Assay for Adult Drosophila
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Cited by 11 •
2016
Taste is an important sensory process which facilitates attraction to beneficial substances and avoidance of toxic substances. This protocol describes a simple ingestion assay for determining Drosophila gustatory preference for a given chemical compound.
08:44
Generation of Topically Transgenic Rats by In utero Electroporation and In vivo Bioluminescence Screening
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Cited by 7 •
2013
Genes can be manipulated during development of the cortex or the hippocampus of the rat via in utero electroporation (IUE) at E16, to enable fast and targeted modifications in neuronal connectivity for later studies of behavior or neuropathology in adult animals. Postnatal in vivo imaging for control of IUE success is performed by bioluminescence of activating co-transfected luciferase.
11:44
Preparing and Administering Topical Medications
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2017
Source: Madeline Lassche, MSNEd, RN and Katie Baraki, MSN, RN, College of Nursing, University of Utah, UT Topical medications are applied directly to the body surfaces, including the skin and mucous membranes of the eyes, ears, nose, vagina, and rectum. There are many classes of topical medications, such as creams, ointments, lotions, patches, and aerosol sprays. Medications that are applied to the skin to produce a slow, controlled, systemic effect are also referred to as transdermal.
FAQs
The cuticle serves as the initial barrier and exposure interface. After researchers place the measured compound on the external surface, penetration through this layer determines how much of the substance can enter the organism and produce an effect. Differences in cuticular penetration therefore help explain variation in physiological responses among tested adults.
Dose-response analysis connects the amount applied to the frequency or severity of observed effects. Testing measured doses allows investigators to determine whether increasing exposure corresponds to outcomes such as mortality, immobilization, or another physiological response. This relationship supports comparisons among candidate compounds and helps characterize relative insecticidal activity under controlled exposure conditions.
Topical exposure places the compound directly on the external surface, whereas oral or environmental routes introduce additional variation associated with ingestion or surrounding conditions. By reducing those sources of exposure variability, the assay helps investigators focus on effects associated with the applied dose and compare compounds more consistently in mature organisms.
A basic workflow uses mature organisms, applies a measured dose directly to each external surface, and maintains the subjects under controlled conditions. Investigators then record defined outcomes, including mortality, immobilization, or other physiological responses, across the tested doses. Comparing these observations produces the dose-response information needed to evaluate activity.
The assay can reveal whether a candidate compound produces mortality, immobilization, or other physiological changes after surface application. When these outcomes are recorded across measured doses, investigators can compare both the presence and degree of activity. Such results provide a controlled basis for screening compounds and assessing their potential effects on mature insects.
Researchers can use the method to screen candidate compounds, compare insecticidal activity, and generate evidence for toxicology studies. Its controlled exposure design also contributes to developing more selective control strategies by clarifying how applied substances affect mature insects. The resulting comparisons can help identify compounds warranting further investigation in pest-management research.