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Neuroscience

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Generation of Topically Transgenic Rats by In utero Electroporation and In vivo Bioluminescence Screening
 

Generation of Topically Transgenic Rats by In utero Electroporation and In vivo Bioluminescence Screening

Article doi: 10.3791/50146
September 24th, 2013

Summary September 24th, 2013

Genes can be manipulated during development of the cortex or the hippocampus of the rat via in utero electroporation (IUE) at E16, to enable fast and targeted modifications in neuronal connectivity for later studies of behavior or neuropathology in adult animals. Postnatal in vivo imaging for control of IUE success is performed by bioluminescence of activating co-transfected luciferase.

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