Aggregation Kinetics
Aggregation Kinetics - Related Videos
06:49
Monitoring Protein Aggregation Kinetics In Vivo using Automated Inclusion Counting in Caenorhabditis elegans
0 Views •
Cited by 2 •
2021
Here, a method is presented for the analysis of protein aggregation kinetics in the nematode Caenorhabditis elegans. Animals from an age-synchronized population are imaged at different time points, followed by semiautomated inclusion counting in CellProfiler and fitting to a mathematical model in AmyloFit.
03:07
Kinetic Molecular Theory: Molecular Velocities, Temperature, and Kinetic Energy
0 Views •
2020
The kinetic molecular theory qualitatively explains the behaviors described by the various gas laws. The postulates of this theory may be applied in a more quantitative fashion to derive these individual laws. Collectively, the molecules in a sample of gas have average kinetic energy and average speed; but individually, they move at different speeds. Molecules frequently undergo elastic collisions in which the momentum is conserved. Since the colliding molecules are deflected off at different...
09:22
In Vitro Aggregation Assays Using Hyperphosphorylated Tau Protein
0 Views •
Cited by 21 •
2015
Unmodified and hyperphosphorylated tau proteins were used in two in vitro aggregation assays to reveal the hyperphosphorylation-dependent fast aggregation kinetics. These assays pave the way for future screens for compounds that can modulate the propensity of hyperphosphorylated tau to form fibrils that underlie the progression of Alzheimer’s disease.
08:07
Enzyme Assays and Kinetics
0 Views •
2023
Enzyme kinetics describes the catalytic effects of enzymes, which are biomolecules that facilitate chemical reactions necessary for living organisms. Enzymes act on molecules, referred to as substrates, to form products. Enzyme kinetic parameters are determined via assays that directly or indirectly measure changes in substrate or product concentration over time. This video will cover the basic principles of enzyme kinetics (including rate equations) and kinetic models. The concepts governing...
10:10
Aggregates for Concrete and Asphaltic Mixes
0 Views •
2023
Source: Roberto Leon, Department of Civil and Environmental Engineering, Virginia Tech, Blacksburg, VA Concrete and asphalt are by far the most common construction materials used today. Concrete is a composite material consisting of cement, water, air, coarse aggregate, and fine aggregates. Fine aggregates are typically sands and coarse aggregates are natural or crushed rocks. Chemical admixtures to modify certain specific properties are also commonly used (i.e., superplasticizers to make the...
FAQs
Two early routes can initiate aggregation: nucleation or collision. In a nucleation pathway, an initial event precedes continued assembly, whereas collision brings dispersed entities together directly. Subsequent growth occurs when additional molecules, particles, or cells bind to the developing assembly. Tracking these stages helps distinguish an initial formation step from later enlargement in a time-course experiment.
Concentration, temperature, pH, ionic strength, and molecular interactions can shift the balance between aggregate formation and dissociation. Because these variables affect association conditions, changing one may alter both the observed rate and the resulting assembly pattern. Comparing aggregation under controlled conditions helps identify which environmental or molecular factors govern behavior in a biological sample.
Observed aggregation reflects competition between formation and dissociation rather than assembly alone. New binding can increase aggregate size or abundance, while dissociation can counteract that increase. Consequently, two samples may show different time courses even when their initial components are similar. Including both processes in interpretation gives a more accurate view of aggregate stability and reaction progress.
Researchers follow reaction progress over time by measuring changes in particle size, scattering, turbidity, or aggregate abundance. These measurements provide time-dependent signals that can be compared across experimental conditions and used to model the course of aggregation. The selected readout should reflect the type of assembly being studied and the feature that changes as aggregation proceeds.
In biopharmaceutical research, time-dependent aggregation measurements help characterize how a formulation behaves under selected conditions. Comparing particle size, scattering, turbidity, or aggregate abundance across conditions can reveal differences in aggregation progress. This information supports formulation optimization by identifying conditions associated with a more favorable balance between aggregate formation and dissociation.
The approach is useful for studying protein misfolding and amyloid formation, where the timing and pathway of assembly are important research questions. It also contributes to understanding cellular or colloidal organization by examining how dispersed components form larger assemblies. In each case, time-resolved measurements connect observed aggregate changes with underlying biological or physicochemical processes.