Antigen Presentation
Antigen Presentation - Related Videos
01:29
Assessing Impaired MHC-I Antigen Presentation in Immune Cells Infected with Bacteria
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2026
Start with an immune cell culture infected with pathogenic bacteria that form intracellular inclusion bodies.The bacterial infection impairs the antigen presentation pathway in the immune cells.Take an uninfected culture as a control.Next, add a model protein antigen and incubate.Immune cells internalize, process, and present the model antigen-derived peptides on MHC-I molecules.After incubation, wash the cells to remove any unprocessed antigen.Fix the cells, then add a quenching agent to...
06:57
Development of an Antigen-driven Colitis Model to Study Presentation of Antigens by Antigen Presenting Cells to T Cells
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Cited by 9 •
2016
In this antigen-driven colitis model, OT-II CD4+ T cells expressing a red fluorescent protein were adoptively transferred into RAG-/- mice that express a green fluorescent protein in mononuclear phagocytes (MPs). The hosts were challenged with Escherichia coli (E.coli) expressing the ovalbumin protein (OVA) fused to a cyan fluorescent protein (CFP).
12:48
Purification of the Membrane Compartment for Endoplasmic Reticulum-associated Degradation of Exogenous Antigens in Cross-presentation
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Cited by 2 •
2017
The method described here is a new vesicle isolation protocol, which allows for the purification of the cellular compartments where exogenous antigens are processed by endoplasmic reticulum-associated degradation in cross-presentation.
18:08
Interview: Glycolipid Antigen Presentation by CD1d and the Therapeutic Potential of NKT cell Activation
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Cited by 2 •
2007
Natural Killer T cells (NKT) are critical determinants of the immune response to cancer, regulation of autioimmunity, clearance of infection, and the development of artheriosclerotic plaques. In this interview, Mitch Kronenberg discusses his laboratory's efforts to understand the mechanism through which NKT cells are activated by glycolipid antigens.
01:25
Self-Presentation
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2025
Self-presentation is a fundamental aspect of social interaction, shaping both how others perceive individuals and how they view themselves. This dynamic process influences behaviors in various social settings, often leading people to adjust their appearance, speech, and demeanor to align with their desired identity. While self-presentation can be deliberate or unconscious, it plays a critical role in interpersonal relationships and self-perception.Forms of Self-PresentationSelf-presentation can...
FAQs
The key distinction is the origin and processing location of the protein material. Intracellular proteins are degraded and loaded onto MHC class I, whereas extracellular proteins are processed in endosomal compartments and loaded onto MHC class II. This division organizes information from different cellular sources for immune recognition and supports distinct downstream immune activities.
A T-cell receptor recognizes a peptide only when it is associated with an MHC molecule on the cell surface. This combined peptide-MHC structure provides the molecular signal that allows T lymphocytes to identify displayed protein fragments rather than simply detecting the original intact protein. Recognition can then initiate cytotoxic activity or helper-cell signaling.
The protein’s location helps determine which processing route supplies the displayed peptide. Material originating inside a cell enters the MHC class I pathway, while material acquired from outside proceeds through endosomal processing and MHC class II presentation. This distinction helps the immune system compare internal cellular information with external material when evaluating potential threats.
A basic analysis follows the information flow from protein source to immune response. First, determine whether the protein is intracellular or extracellular. Next, examine its degradation or endosomal processing and the associated MHC class. Finally, assess recognition of the peptide-MHC complex by T lymphocytes and record whether the outcome involves cytotoxic activity or helper-cell signaling.
It is especially relevant when researchers examine how immune responses arise during infection, autoimmunity, vaccination, transplantation, or cancer. In each setting, the displayed peptide-MHC complexes provide a way to connect protein processing with T-cell recognition. Studying that connection can clarify immune behavior and support development of immunotherapies or diagnostic approaches.
In vaccine research, antigen presentation provides a framework for examining how protein-derived fragments become available for T-cell recognition. In cancer immunology, the same framework helps investigate immune detection of abnormal tissue. Across both areas, analyzing peptide-MHC display and subsequent T-cell signaling connects molecular processing with potential immune responses and therapeutic strategies.