Antiseptics
Antiseptics - Related Videos
06:19
Decontamination for Laboratory Biosafety
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2023
Robert M. Rioux and Zhifeng Chen, Pennsylvania State University, University Park, PA Decontamination is essential for laboratory biosafety, as the accumulation of microbial contamination in the laboratory can lead to the transmission of disease. The degree of decontamination can be classified as either disinfection or sterilization. Disinfection aims to eliminate all pathogenic microorganisms, with the exception of bacterial spores on lab surfaces or equipment. Sterilization, on the other hand,...
01:27
Compound Administration in Rabbits II
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2026
All animal procedures described here must be conducted in accordance with institutional animal ethics guidelines and approved by IACUC. All procedures must follow the principles of the 3Rs—Replacement, Reduction, and Refinement—and must be performed by trained personnel. Compounds can be administered to rabbits by subcutaneous, intramuscular, or intravenous injection depending on the study objectives, required absorption profile, and characteristics of the test article. Prior to administration,...
07:44
Surgical Injury to the Mouse Pancreas through Ligation of the Pancreatic Duct as a Model for Endocrine and Exocrine Reprogramming and Proliferation
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Cited by 19 •
2015
This protocol describes an injury model involving the surgical ligation of the pancreatic duct in the adult mouse pancreas, resulting in severe injury that establishes an environment that allows beta cell neogenesis and proliferation. This model can be used as a tool to study mechanisms involved in beta cell formation.
07:35
Creating Rigidly Stabilized Fractures for Assessing Intramembranous Ossification, Distraction Osteogenesis, or Healing of Critical Sized Defects
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Cited by 20 •
2012
This article describes a method for stabilizing long bone fractures that is based on the application of modified Ilizarov external fixators 1-3. After application of the fixators and creation of the bone injury, healing can be assessed, distraction osteogenesis can be performed, or non-union or critical sized defect can be created and used to study therapeutic interventions.
07:43
Endoscopic Endonasal Trans-sphenoidal Approach: Minimally Invasive Surgery for Pituitary Adenomas
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Cited by 18 •
2018
The aim of this paper is to describe the different steps of the endoscopic endonasal approach to the sella turcica.
FAQs
Their effects depend on the chemical compound and its target within the microorganism. Some disrupt cell membranes, causing loss of cellular integrity; others denature proteins, oxidize cellular components, or damage nucleic acids. These mechanisms interfere with essential microbial structures and processes, producing inhibition or reduction of microorganisms on treated tissue.
Antiseptic compounds do not act through a single universal mechanism. One agent may primarily affect membranes, while another denatures proteins, oxidizes cellular components, or damages nucleic acids. Consequently, the biological outcome depends on the compound selected and the susceptibility of the microorganisms present, making mechanism an important consideration in infection-control decisions.
Concentration, contact time, and microorganism type are central variables. A treatment must provide an appropriate concentration and remain in contact with tissue long enough to act, while the organisms present may differ in susceptibility. These factors help explain why the same antiseptic procedure can produce different results under different biological or practical conditions.
Selection requires balancing microbial reduction with protection of host tissues. The intended setting, such as wound care, surgical skin preparation, hand hygiene, or another infection-control procedure, should be considered alongside the compound’s mechanism, concentration, contact time, and the microorganisms involved. This approach helps address microbial contamination without overlooking tissue safety or procedural limitations.
They support several infection-control procedures by treating living tissue or skin in settings where reducing microorganisms is important. Applications include wound care, preparation of skin before surgery, and hand hygiene. In each context, effectiveness depends on using an appropriate agent under suitable concentration and contact-time conditions while maintaining biological safety for the treated tissue.
Appropriate use can reduce or inhibit microorganisms on living tissue, helping prevent infection and limit microbial transmission. Its value is therefore assessed not only by immediate microbial reduction but also by whether the procedure supports safe wound management, surgical preparation, hand hygiene, or broader infection control. Limitations must be considered when interpreting the outcome.