Apertureless Near Field Microscope
Apertureless Near Field Microscope - Related Videos
12:27
Assembly, Tuning and Use of an Apertureless Near Field Infrared Microscope for Protein Imaging
0 Views •
Cited by 3 •
2009
The assembly of a nearfield infrared microscope for imaging protein aggregates is described.
06:48
A Multimodal Wide-Field Fourier-Transform Raman Microscope
0 Views •
2025
A wide-field Fourier-transform microscope, based on a compact and ultra-stable birefringent interferometer, allows the parallel acquisition of spectra for all pixels of a 2D detector. The time-domain approach enables the disentanglement of photoluminescence and Raman signals, and allows rapid Raman mapping (~5 ms/pixel) with ~1-µm spatial and 23-cm-1 spectral resolution.
08:53
Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope
0 Views •
Cited by 15 •
2014
A module for single plane illumination microscopy (SPIM) is described which is easily adapted to an inverted wide-field microscope and optimized for 3-dimensional cell cultures. The sample is located within a rectangular capillary, and via a microfluidic system fluorescent dyes, pharmaceutical agents or drugs can be applied in small quantities.
08:08
Major Components of the Light Microscope
0 Views •
2008
The light microscope is a basic tool for the cell biologist, who should have a thorough understanding of how it works, how it should be aligned for different applications, and how it should be maintained as required to obtain maximum image-forming capacity and resolution. The components of the microscope are described in detail here.
08:13
A Guide to Build a Highly Inclined Swept Tile Microscope for Extended Field-of-view Single-molecule Imaging
0 Views •
Cited by 2 •
2019
A detailed instruction is described on how to build a highly inclined swept tile (HIST) microscope and its usage for single-molecule imaging.
FAQs
A sharp probe creates a nanoscale site where the incident light interacts strongly with the sample. The resulting local optical field is enhanced and scattered by the probe-sample interaction, so the measured signal can vary over distances smaller than the conventional diffraction limit. This localized response enables optical mapping alongside nanoscale positioning.
A metal-coated or metallic tip participates directly in the local light-matter interaction at the sample surface. Its presence enhances the local optical field and produces scattered light that carries information about the nearby material. Monitoring this scattering makes it possible to connect nanoscale probe locations with local optical and chemical differences.
The atomic force microscope controls the probe position while the optical system monitors scattering from the illuminated tip and sample region. Because both signals are collected during scanning, researchers can compare surface morphology with local optical response. This paired measurement helps distinguish structural variations from changes in the material’s optical or chemical behavior.
The process begins by illuminating a metal-coated or metallic tip positioned near the sample. The atomic force microscope then controls the probe as it scans relevant locations, while the system monitors light scattered through the tip-sample interaction. The resulting measurements are organized into topographic and optical maps for spatial comparison.
In biological research, the method can examine membranes, proteins, and other cellular components where chemical or structural variation occurs at nanometer-scale locations. Its value comes from examining these features together with their surface morphology. This supports analysis of molecular organization and heterogeneous regions that may not be uniform across a biological sample.
Spectroscopic information adds a chemical dimension to the structural and topographic measurements. In biological systems, that combination can help investigate molecular organization, interactions, and spatial heterogeneity within membranes, proteins, and cellular components. Researchers can therefore relate a local optical response to the physical location and morphology of the feature being studied.