Atp Bioluminescence Assay
Atp Bioluminescence Assay - Related Videos
08:32
The ATP Bioluminescence Assay
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2023
In fireflies, the luciferase enzyme converts a compound called luciferin into oxyluciferin, and produces light or “luminescence” as a result. This reaction requires energy derived from ATP in order to proceed, so researchers have exploited the luciferase-luciferin interaction to gauge ATP levels in cells. Given ATP’s role as the cell’s currency of energy, the ATP bioluminescence assay can provide insight into cellular metabolism and overall cell health.In this video, JoVE discusses cellular...
08:05
Assaying Protein Kinase Activity with Radiolabeled ATP
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Cited by 15 •
2017
Protein kinases are highly evolved signaling enzymes and scaffolds that are critical for inter- and intracellular signal transduction. We present a protocol for measuring kinase activity through the use of radiolabeled adenosine triphosphate ([γ-32P] ATP), a reliable method to aid in elucidation of cellular signaling regulation.
03:17
Cellular Bioluminescence Based Assay: A High Throughput Method for Combinatorial Drug Screening
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2023
In this video, we describe a method highlighting the utility of bioluminescence assay for sensitive and rapid screening of drugs. These drugs can be tested alone or in combination to assess the synergism in their mode of action.
02:56
An ATP Utilization Assay for Determining Bacterial Survival Under Antibiotics Treatment
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2025
This video describes the simple ATP-measuring assay using luciferase glow reagent. This assay is used to quantify Neisseria gonorrhoeae survival after treatment with ceftriaxone.
01:31
ATP Yield
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2019
Cellular respiration produces 30 - 32 ATP per glucose molecule. Although most of the ATP results from oxidative phosphorylation and the electron transport chain (ETC), 4 ATP are gained beforehand (2 from glycolysis and 2 from the citric acid cycle). The ETC is embedded in the inner mitochondrial membrane and is comprised of four main protein complexes and an ATP synthase. NADH and FADH2 pass electrons to these complexes, which pump protons into the intermembrane space. This distribution of...
FAQs
ATP drives the luciferin-luciferase reaction, causing the chemical process to produce photons. The assay therefore converts the presence of cellular ATP into a light signal that can be measured. Because ATP is the primary energy-carrying molecule in living cells, this reaction provides a biological readout that can support estimates of cellular or microbial activity.
Photon intensity generally reflects the amount of ATP present in the sample. A stronger measured light signal therefore indicates more ATP under the assay conditions, while a weaker signal indicates less. This relationship allows researchers to quantify the assay output and use it as an estimate of biological activity, cell viability, or related changes in the sample.
The luciferin-luciferase reaction produces photons that are not interpreted by visual inspection alone. A luminometer quantifies the emitted light and provides the measurement used for analysis. Its role makes the assay suitable for rapid, sensitive comparisons among cultured cells, tissues, microbial samples, or environmental samples with different ATP-associated signals.
This approach can be applied to cultured cells, tissues, and environmental samples. That range supports different biological questions, including how many viable cells remain after treatment, whether microbial contamination is present, or whether a sample shows measurable biological activity. The same light-based readout can therefore serve both laboratory biology and monitoring applications.
A typical measurement begins with a sample containing cellular or microbial ATP, followed by exposure to the luciferin-luciferase reaction. The resulting photons are then quantified with a luminometer, and the light intensity is interpreted as generally reflecting the sample's ATP amount. Researchers can compare these measurements to estimate differences in viability, proliferation, contamination, or activity.
In cultured-cell experiments, ATP measurements can help estimate cell viability and proliferation after a treatment. Cytotoxicity testing uses changes in the ATP-associated light signal to evaluate biological effects, while drug-development studies can apply the same readout when comparing responses across experimental conditions. The rapid, sensitive format supports efficient assessment of treatment-related changes.
Microbial cells contribute ATP to a sample, so measuring the associated bioluminescent signal can help estimate microbial contamination or biological activity. This makes the assay useful for microbial detection and hygiene monitoring, including environmental samples. Its rapid and sensitive measurement format allows researchers or monitoring programs to identify differences in ATP-associated signals across samples.