Mouse Development
Mouse Development - Related Videos
09:04
Development and Reproduction of the Laboratory Mouse
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2023
Successful breeding of the laboratory mouse (Mus musculus) is critical to the establishment and maintenance of a productive animal colony. Additionally, mouse embryos are frequently studied to answer questions about developmental processes. A wide variety of genetic tools now exist for regulating gene expression during mouse embryonic and postnatal development, which can help scientists to understand more about heritable diseases affecting human development. This video provides an introduction...
05:53
In vivo Electroporation of Developing Mouse Retina
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Cited by 33 •
2011
A method for the incorporation of plasmid DNA into murine retinal cells for the purpose of performing either gain- or loss of function studies in vivo is presented. This method capitalizes on the transient increase in permeability of cell plasma membranes induced by the application of an external electrical field.
08:51
Evaluation of Mammary Gland Development and Function in Mouse Models
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Cited by 98 •
2011
This method describes how to dissect and assess mammary gland development and function from mice. Excised mammary glands are assessed for the degree of development using whole mount while milk ejection is evaluated using an oxytocin-based myoepithelial cell contraction assay.
07:20
Cell Labeling and Injection in Developing Embryonic Mouse Hearts
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Cited by 3 •
2014
We describe a series of methods to inject dyes, DNA vectors, virus, and cells in order to monitor both cell fate and phenotype of endogenous and grafted cells derived from embryonic or pluripotent cells within mouse embryos at embryonic day (E)9.5 and later stages of development.
11:48
Genetic Manipulation of the Mouse Developing Hypothalamus through In utero Electroporation
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Cited by 14 •
2013
Despite the functional and medical importance of the hypothalamus, in utero genetic manipulation of its development has rarely been attempted. We show a detailed procedure for in utero electroporation into the mouse hypothalamus and show representative results of total and partial (regional) hypothalamic transfection.
FAQs
Gene regulation controls when developmental genes are active, while signaling pathways help cells respond to positional and environmental cues. Together, these systems coordinate cell division, differentiation, migration, and tissue patterning. Their interaction establishes the body plan and guides the formation of organ systems, making them central subjects in studies of developmental biology and genetic disease.
These processes contribute different but connected functions. Cell division increases cell number, differentiation gives cells specialized identities, migration positions cells within the embryo, and tissue patterning organizes them into structures. Coordinated timing and interaction among these activities allow the developing mouse to establish body organization and form functional organ systems rather than simply accumulate cells.
Many mechanisms that guide mouse development are conserved among mammals. This conservation allows researchers to use findings from mouse embryos and postnatal animals to inform questions about human development, while still examining how gene regulation and signaling shape tissues in a living mammalian system. The approach also supports research connecting developmental processes with regenerative medicine.
Researchers examine both embryonic and postnatal development to follow changes from early body-plan formation through later growth. Comparing these periods helps investigators relate gene regulation, signaling, tissue patterning, and organ-system formation to developmental outcomes. This broad time frame is useful when studying normal biology as well as changes caused by genetic or environmental factors.
Mouse development models can be used to investigate how genetic changes affect growth, tissue organization, and organ-system formation. They also support studies of congenital disorders and the developmental consequences of environmental changes. By connecting altered conditions with developmental outcomes, researchers can examine relationships between biological mechanisms and disease-related phenotypes.
Studies of mouse development reveal how cells acquire identities, move into organized tissues, and participate in organ-system formation. Because many underlying mechanisms are conserved among mammals, these findings can inform regenerative medicine research. Developmental knowledge provides a biological context for understanding how tissues are established and how related processes might guide efforts to restore or replace damaged structures.