Btx Electroporation System
Btx Electroporation System - Related Videos
19:04
In Utero Intraventricular Injection and Electroporation of E16 Rat Embryos
0 Views •
Cited by 13 •
2007
In-utero in-vivo injection and electroporation of the embryonic rat neocortex provides a powerful tool for the manipulation of individual progenitors ...
11:57
Electroporation of Mycobacteria
0 Views •
Cited by 55 •
2008
Mycobacterial pathogenic strategies remain poorly understood. The slow growth rate of most species, the impenetrable nature of the cell-wall, and the hazards of working with pathogens make mycobacteria difficult to study and are largely responsible for our poor understanding of these organisms. In this video we will demonstrate the technique of electroporation, which involves subjecting cells to a brief high electrical impulse to allow the entry of DNA. It is the most widely used method for...
26:17
In Utero Intraventricular Injection and Electroporation of E15 Mouse Embryos
0 Views •
Cited by 52 •
2007
In-utero in-vivo injection and electroporation of the embryonic mouse neocortex provides a powerful tool for the manipulation of individual progenitors ...
08:05
Murine In Utero Electroporation
0 Views •
2023
In utero electroporation is an important technique for studying the molecular mechanisms that guide the proliferation, differentiation, migration, and maturation of cells during neural development. Electroporation enables the rapid and targeted delivery of material into cells by utilizing electrical pulses to create transient pores in cell membranes. Although electroporation has traditionally been used in in vitro studies, scientific advancements have now broadened its utilization to intact...
12:19
Bacterial Transformation: Electroporation
0 Views •
2023
The term “transformation” refers cellular ingestion of foreign DNA. In nature, transformation can occur in certain types of bacteria. In molecular biology, however, transformation is artificially induced through the creation of pores in the bacterial cell walls. Bacterial cells that are able to take up DNA from the environment are called competent cells. Electrocompetent cells can be produced in the laboratory and transformation of these cells can be achieve via the application of an...
FAQs
A brief electric field temporarily disrupts the cell membrane, creating transient pores through which cargo can pass. The membrane then reseals, so delivery depends on producing sufficient permeability without compromising cell viability. This balance is central to using the system for introducing DNA, RNA, proteins, or other molecules into living cells.
Pulse conditions influence both cargo entry and the condition of the treated cells. Researchers can adjust these conditions to improve delivery while maintaining viability, rather than applying one fixed setting to every experiment. This flexibility supports different objectives, including genetic material delivery, gene expression studies, functional genomics, and gene-editing workflows.
The system provides an alternative to chemical and viral approaches for introducing molecules into cells. Its distinguishing basis is a controlled electrical pulse that temporarily opens membrane pores, rather than relying on chemical treatment or viral delivery. This makes electroporation relevant when researchers need a nonchemical, nonviral route for cellular transfection and related studies.
The approach can deliver several classes of biological cargo, including DNA, RNA, proteins, and other molecules. This range allows the same general system to support different experimental aims rather than limiting work to a single nucleic-acid type. The selected cargo can be used in transfection, gene expression, functional genomics, or gene-editing workflows.
A basic experiment brings living cells and the selected cargo together, applies a controlled electrical pulse, and allows the membrane to reseal after transient pore formation. Researchers then use the treated cells for the intended biological study, while considering whether the pulse conditions supported delivery and preserved viability. The workflow therefore links electrical treatment with downstream cellular analysis.
In biology and biotechnology, the system supports cellular transfection, gene expression studies, functional genomics, and gene-editing workflows. These applications use delivered genetic material or other molecules to examine cellular responses and biological function. Because pulse conditions can be adjusted, researchers can tailor delivery experiments toward effective molecular entry while retaining viable cells for subsequent investigation.