Cell Cycle Analysis
Cell Cycle Analysis - Related Videos
09:32
Cell Cycle Analysis
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2023
Cell cycle refers to the set of events through which a cell grows, replicates its genome, and ultimately divides into two daughter cells through the process of mitosis. Because the amount of DNA in a cell shows characteristic changes throughout the cycle, techniques known as cell cycle analysis can be used to separate a population of cells according to the different phases of cell cycle they are in, based on their varying DNA content.This video will cover the principles behind cell cycle...
12:19
Analysis of Cell Cycle Position in Mammalian Cells
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Cited by 71 •
2012
Determining the cell cycle position of a population of cells, or understanding how signals affect proliferation, can be readily measured by flow cytometry using this protocol. We report a simple experimental approach to staining cells and quantifying their position in the cell cycle.
03:34
Cell Cycle Analysis: An Approach to Study Cell Cycle Regulation of miRNA-transfected Lung Cancer Cells
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2023
miRNA or microRNA are key regulators of cell cycle progression. This video describes a flow cytometry-based cell cycle analysis of lung cancer cells transfected with miRNA, to study the effect of miRNA on cell cycle regulation. Inhibition of proliferation by halting cell cycle progression has shown potential to be a promising approach for cancer treatment.
06:20
An Assay for Cell Cycle Analysis of Antigen-Specific CD8+ T Cells
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2025
This video demonstrates an assay to analyze antigen-specific CD8+ T cells in different cell cycle stages. Fluorescently-labeled viable cells are selected using flow cytometry, and the cells in the different cell cycle stages are identified by their DNA content using antibodies against cell proliferation-associated nuclear protein and a nucleic acid stain.
08:13
Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast
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2025
This protocol details two methods of yeast cell cycle arrest and optional release, and elaborates on the use of fluorescence microscopy to study cell cycle-dependent processes in S. cerevisiae.
FAQs
DNA-content staining separates cell-cycle populations because DNA amount changes as cells replicate their genomes. Cells in G0/G1 have an initial DNA content, S-phase cells show intermediate content while replication is underway, and G2/M cells have completed replication and therefore contain the higher content. Flow-cytometric measurement converts these differences into population-level patterns that indicate how cells are distributed across the cycle.
An incorporated nucleotide analog identifies cells actively synthesizing DNA, making it an indicator of replication activity within S phase. DNA-content measurements show where cells fall according to total DNA, whereas analog incorporation highlights ongoing synthesis. Using the two readouts together can distinguish a broad S-phase population from evidence that cells are actively duplicating their genomes.
Mitotic proteins provide markers for cells undergoing division, complementing DNA-content measurements that group cells into G0/G1, S, and G2/M populations. Their presence helps identify the mitotic portion of the high-DNA-content group rather than treating all G2/M cells as equivalent. This distinction is useful when a biological change affects entry into, progression through, or completion of mitosis.
Researchers typically measure cellular DNA content with a stain and analyze the resulting signal by flow cytometry. The data are used to distinguish G0/G1, S, and G2/M populations. Depending on the question, incorporated nucleotide analogs can report DNA synthesis, while mitotic proteins can identify cells in division. Comparing these measurements across conditions reveals shifts in cell-cycle progression.
It is useful when researchers need to determine whether stress, a drug treatment, or a genetic change alters proliferation or produces cell-cycle arrest. A shift in the relative populations, together with DNA-synthesis or mitotic-marker measurements, can show where progression is affected. This makes the approach valuable for connecting an experimental condition with changes in cell-cycle control rather than measuring proliferation alone.
It links population-level cell-cycle patterns to biological processes including development, cancer, and tissue maintenance. The measurements can also support investigation of genome duplication and the control of cell division, especially when cells respond differently to stress or genetic change. Thus, the method provides experimental context for asking why proliferation changes and which stage-associated process may be involved.