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Chapters
- 00:05Title
- 01:19Labeling and Fixing of Cells
- 02:30Denaturing and Staining of Brdu and DNA
- 04:11Flow Cytometry and Data Analysis
- 06:10Alternate Protocol for Cell Cycle Analysis in a Mixed Population of Cells
- 07:35Cell Cycle Distribution Staining Can Be Used for a Variety of Experimental Purposes
- 11:47Conclusion
Determining the cell cycle position of a population of cells, or understanding how signals affect proliferation, can be readily measured by flow cytometry using this protocol. We report a simple experimental approach to staining cells and quantifying their position in the cell cycle.
