Cell Surface Expression
Cell Surface Expression - Related Videos
04:55
Assaying Surface Expression of Chemosensory Receptors in Heterologous Cells
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Cited by 6 •
2011
Here we demonstrate a protocol to carry out live cell staining that can be used to detect odorant receptors on the surface of HEK293T cells conveniently. In addition, it may also be used to assay for surface expression of other chemosensory receptors or GPCRs.
11:32
Determination of the Relative Cell Surface and Total Expression of Recombinant Ion Channels Using Flow Cytometry
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Cited by 11 •
2016
Inherited cardiac arrhythmias are often caused by mutations that alter the surface delivery of one or more ion channels. Here, we adapt flow cytometry assays to provide a quantification of the relative total and cell surface protein expression of recombinant ion channels expressed in tsA-201 cells.
10:42
Determining Cell-surface Expression and Endocytic Rate of Proteins in Primary Astrocyte Cultures Using Biotinylation
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Cited by 6 •
2017
Two biotinylation-based methods, designed for determining the cell-surface expression and endocytic rate of proteins expressed at the plasma membrane, are presented in this report.
04:39
Wireless Surface Electromyography Recording of Facial Muscle Activity During Expressions
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2025
Source:Hila Man1, Paul F. Funk2, Ieva Vebraite-Adereth3, Christoph Anders4, Orlando Guntinas-Lichius2, Yael Hanein1,3,51Sagol School of Neuroscience, Tel Aviv University, 2Department of Otorhinolaryngology, Jena University Hospital, Friedrich-Schiller-University Jena, 3School of Electrical Engineering, Tel Aviv University, 4Division Motor Research, Pathophysiology and Biomechanics, Department of Trauma, Hand and Reconstructive Surgery, Jena University Hospital, Friedrich-Schiller-University...
08:49
Cell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens
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Cited by 8 •
2020
This manuscript describes a genome-scale cell-based screening approach to identify extracellular receptor-ligand interactions.
FAQs
Newly synthesized membrane-associated molecules move through the endoplasmic reticulum and Golgi apparatus, where they are processed and sorted before transport. Vesicles then carry selected proteins, lipids, or carbohydrates toward the plasma membrane. This organization links intracellular trafficking with the appearance of specific molecules at the cell boundary and helps determine which signals or interactions the cell can present externally.
Surface abundance reflects a balance between delivery to the plasma membrane, removal by endocytosis, and return through recycling pathways. Increased delivery or recycling can raise the amount available for communication, whereas greater internalization can reduce it. This balance allows cells to adjust receptor signaling, adhesion, antigen display, and transport functions without relying only on new synthesis.
A molecule can influence cell behavior when it is accessible at the plasma membrane, because that location enables interactions with the surrounding environment. Surface-localized receptors can participate in signaling, adhesion molecules can support cell interactions, and displayed antigens can contribute to immune recognition. Consequently, altered trafficking or membrane abundance may change cellular responses even when the relevant molecule is produced inside the cell.
Developmental cues, immune activation, disease-associated mutations, and therapeutic treatments can alter the trafficking or turnover of molecules at the plasma membrane. Such changes may affect delivery through the endoplasmic reticulum and Golgi apparatus, vesicle transport, endocytosis, or recycling. Measuring the resulting surface pattern helps connect an external or genetic perturbation with changes in cellular communication and function.
A study can assess changes in the molecules presented at the plasma membrane and relate them to their trafficking through the endoplasmic reticulum, Golgi apparatus, vesicles, and recycling pathways. The comparison may focus on conditions such as developmental cues, immune activation, mutations, or treatment. Resulting measurements can indicate altered receptor signaling, adhesion, antigen display, or membrane transport.
This analysis is useful when researchers need to determine how cells communicate, attach to one another, display antigens, or move substances across the membrane. It also provides context for studying developmental regulation, immune responses, disease-associated mutations, and therapeutic effects. Examining surface changes can therefore connect membrane trafficking with observable differences in cellular behavior and environmental responsiveness.