Commercial Gel Extraction Kits
Commercial Gel Extraction Kits - Related Videos
11:03
Pouring and Running a Protein Gel by reusing Commercial Cassettes
0 Views •
Cited by 2 •
2012
Our protocol demonstrates how to pour multiple protein gels at a time by recycling Invitrogen Nupage Novex minigel cassettes, and inexpensive materials purchased at a home improvement store. This economical and streamlined method includes a way to store the gels at 4°C for a few weeks. By re-using the plastic gel cassettes from commercially available gels, labs that run frequent protein gels can save significant costs and help the environment.
17:25
RNA Extraction from Neuroprecursor Cells Using the Bio-Rad Total RNA Kit
0 Views •
Cited by 1 •
2007
12:39
Rapid Genotyping of Mouse Tissue Using Sigma's Extract-N-Amp Tissue PCR Kit
0 Views •
Cited by 4 •
2008
The complete genotyping of a mouse tail sample, including tissue digestion and PCR readout, is done in one and a half hours using Sigma's SYBR Green Extract-N-Amp Tissue PCR kit.
10:43
A Practical and Novel Method to Extract Genomic DNA from Blood Collection Kits for Plasma Protein Preservation
0 Views •
Cited by 4 •
2013
We are describing a new method of isolating genomic DNA from whole blood collected for plasma/serology. After plasma collection, the compacted blood is usually discarded. Our novel method represents a significant improvement over existing methods and makes DNA and plasma available from a single collection, without requesting additional blood.
03:22
SDS-PAGE Based Extraction of Extracellular Vesicles Associated Proteins: A Procedure to Extract Proteins from EVs and Prepare Them for In-Gel Digestion
0 Views •
2023
In this video, we demonstrate the SDS-PAGE in-gel digestion method for protein extraction from extracellular vesicles or EVs. Once isolated, the obtained protein fragments can be used for proteomic analysis.
FAQs
The chaotropic buffer dissolves the excised gel slice and creates conditions that allow DNA to bind to the kit’s silica membrane. This step separates the nucleic acid from the gel matrix while preparing it for washing. Effective dissolution is therefore central to transferring the selected DNA fragment from the solid gel into a purifiable form.
A silica membrane provides a temporary binding surface for DNA during purification. After the dissolved gel mixture is applied, washing removes salts and other contaminants while the DNA remains associated with the membrane. A low-salt buffer or water then releases the fragment, producing material suitable for subsequent molecular biology workflows.
The excised region determines which separated DNA fragment enters purification. Because gel extraction can be performed from either agarose or polyacrylamide gels, the approach connects prior gel separation with recovery of a selected band rather than the entire sample. This selection is important when a downstream experiment requires a particular fragment for analysis or construction.
A typical workflow starts by excising the desired DNA-containing gel slice and dissolving it in chaotropic buffer. The resulting solution is transferred to a silica membrane, where DNA binds. Washing removes salts and contaminants, and a final elution with low-salt buffer or water recovers the purified fragment for later use.
Gel extraction is useful when cloning or sequencing requires a selected DNA fragment rather than an unfractionated mixture. It can also prepare PCR products for downstream work and support restriction-fragment analysis. By removing gel-associated contaminants and excess salts, the kit workflow helps produce DNA in a form compatible with these applications.
Commercial kits standardize the purification format, reducing handling time compared with less standardized processing. Their binding, washing, and elution stages are organized around producing purified DNA from a selected gel fragment. The resulting material can then support cloning, sequencing, PCR product preparation, or restriction-fragment analysis, depending on the experimental objective.