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Chapters
- 00:01Title
- 01:42Protocol for DNA Extraction from P100 Tubes
- 03:13Lyse Red Blood Cells
- 03:56Precipitate Protein
- 04:13Prepare Isopropanol Tubes
- 05:40Resuspend DNA in DNA Hydration Buffer
- 06:33Representative Results
- 08:20Comparison to Alternative Methods
- 09:45Conclusion
We are describing a new method of isolating genomic DNA from whole blood collected for plasma/serology. After plasma collection, the compacted blood is usually discarded. Our novel method represents a significant improvement over existing methods and makes DNA and plasma available from a single collection, without requesting additional blood.
Tags
Genomic DNA ExtractionBlood Collection KitsPlasma Protein PreservationLaboratory TestsBlood Collection TubesDNA AnalysisGenetic AnalysisGenomic AnalysisAnticoagulated Whole BloodEDTA-containing VacutainerHeparin-containing TubeSimultaneous AnalysisParallel AnalysisProteomic DataSingle Tube Collection MethodCompacted BloodSample ProcessingPatient SamplesBD P100 Blood Collection System
