Core Punching
Core Punching - Related Videos
02:10
The Nucleosome Core Particle
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Nucleosomes are the DNA-histone complex, where the DNA strand is wound around the histone core. The histone core is an octamer containing two copies of H2A, H2B, H3, and H4 histone proteins. The paradox Nucleosomes, paradoxically, perform two opposite functions simultaneously. On the one hand, their main responsibility is to protect the delicate DNA strands from physical damage and help achieve a higher compaction ratio. While on the other hand, they must allow polymerase enzymes to access DNA...
02:20
Generating an Excisional Skin Wound in a Mouse Model via Punch Biopsy
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This video demonstrates a punch biopsy procedure to create a precise wound in a mouse model. The excision exposes the subcutaneous layer of the skin, providing a suitable site for studying immune responses to localized pathogen infection.
08:30
Preparation of Formalin-fixed Paraffin-embedded Tissue Cores for both RNA and DNA Extraction
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Cited by 53 •
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02:23
Visualizing the Effect of pH on Solubilization of the Influenza A Viral Core
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Source: Stauffer, S. et al., In Vitro Disassembly of Influenza A Virus Capsids by Gradient Centrifugation. J. Vis. Exp. (2016)This video demonstrates the effect of pH on the solubilization of influenza A viral cores. Gel electrophoresis and protein staining reveal the progressive disassembly of the core structure under acidic conditions.
13:23
A Protocol for Collecting and Constructing Soil Core Lysimeters
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FAQs
Accurate alignment with the selected tissue region determines whether the extracted core represents the intended morphology or biomarker area. The hollow punch or biopsy needle must be positioned over a defined region before being driven through the specimen. Consistent placement helps produce uniform cores and supports reliable comparison among multiple tissue samples.
Preserving the surrounding material allows the remaining specimen to undergo additional processing, sectioning, or analysis after a selected region has been removed. This is particularly valuable when tissue is limited or contains several areas of biological interest. The approach therefore supports tissue conservation while retaining access to morphology and disease-associated changes outside the sampled core.
Cores from different specimens or regions can be placed into a common recipient block for tissue microarray construction. Examining them under consistent staining and imaging conditions reduces variation caused by separate processing environments. This organization makes it easier to compare tissue morphology, protein expression, biomarkers, and disease-associated changes across the selected samples.
A workflow begins by identifying and marking the tissue region of interest. The hollow punch or biopsy needle is aligned with that area and driven through the specimen to capture a cylindrical core. The core can then be transferred for processing, sectioning, or placement in a recipient block, depending on the planned analysis.
Researchers may use the technique when they need to select defined tissue regions for organized comparative analysis while conserving the larger specimen. In histology and pathology, it is useful for assembling tissue microarrays that contain many specimens or regions. These arrays support parallel examination of morphology, protein expression, biomarkers, and disease-related tissue changes.
Prepared tissue cores can support examination of cellular and structural morphology, protein expression, biomarker distribution, and disease-associated changes. Because multiple cores can be examined under consistent staining and imaging conditions, the resulting data are suited to comparisons among specimens or selected tissue regions. Interpretation depends on the biological features retained within each sampled core.