Bead-based Immunoassay
Bead-based Immunoassay - Related Videos
03:45
A Bead-Based Multiplex Immunoassay to Identify Pathogen-Specific Antibodies in Saliva
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2025
This video demonstrates a bead-based multiplex immunoassay technique to simultaneously detect antibodies for multiple environmental pathogens in human saliva. Fluorescent beads were coupled to antigens from different pathogens, which were captured using antibodies in the human saliva sample. Upon labeling the antibodies using a fluorescent reporter, the beads were analyzed using flow cytometry to assess the presence of different pathogen-specific antibodies in the saliva.
04:49
A Multiplex Bead-Based Immunoassay to Quantify Multiple Cytokine Targets
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2025
This video demonstrates a multiplex bead-based immunoassay for quantifying multiple cytokine targets in a sample. A mixture of distinct microspheres conjugated to cytokine-specific antibodies is used to bind the cytokines present in the sample. Streptavidin-tagged reporters are immobilized on the bound-cytokines using biotinylated detection antibodies. Flow cytometry identifies the bound cytokines based on the unique sizes and internal fluorescence intensities of the microspheres, while the...
08:05
Multiplexed Fluorometric ImmunoAssay Testing Methodology and Troubleshooting
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Cited by 19 •
2011
Using Luminex Corporation’s xMAP microsphere technology, we have developed the Multiplexed Fluorometric ImmunoAssay (MFIA) for serosurveillance of various laboratory animal species. The MFIA is a suspension microarray where antigen, tissue control or immunoglobulins are covalently linked to color-coded polystyrene microspheres. The MFIA testing method as well as various troubleshooting topics is addressed.
11:00
Multiplex Cytokine Profiling of Stimulated Mouse Splenocytes Using a Cytometric Bead-based Immunoassay Platform
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Cited by 30 •
2017
This protocol describes the quantification of multiple cytokine targets simultaneously in tissue culture supernatants collected from stimulated mouse splenocytes using multiplex bead based immunoassay platform and a flow cytometer.
09:08
Developing a Salivary Antibody Multiplex Immunoassay to Measure Human Exposure to Environmental Pathogens
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Cited by 11 •
2016
In the current climate of scarce resources, new technologies are emerging that allow researchers to conduct studies cheaper, faster and with more precision. Here we describe the development of a bead-based salivary antibody multiplex immunoassay to measure human exposure to multiple environmental pathogens simultaneously.
FAQs
The capture antibody first provides the binding site for the target molecule in the biological sample. A labeled detection antibody then binds the captured target, creating an immune complex associated with the bead. Fluorescent or enzymatic activity from that label produces the assay signal, allowing the presence or measured amount of the target to be assessed.
Different bead populations can carry different capture antibodies, so each population is associated with a particular analyte. When combined in one sample, they support simultaneous analysis of several proteins, hormones, pathogens, or other targets. This multiplex arrangement broadens the information obtained from a single assay while reducing the need to analyze each analyte separately.
The detection format depends on the label attached to the detection antibody. Fluorescent labels produce an optical signal, whereas enzymatic labels generate a measurable response through enzyme activity. Both formats connect detection-antibody binding to an analytical readout, enabling the assay to identify and measure target molecules in biological samples.
A typical workflow begins with antibody-coated beads contacting the biological sample, allowing target molecules to bind to the capture antibodies. A labeled detection antibody is then introduced so it can form an immune complex with the captured target. The resulting fluorescent or enzymatic signal is measured to determine the assay outcome for the selected analyte or analytes.
Researchers may select this approach when they need sensitive, high-throughput measurement of multiple biological targets or when sample and reagent quantities are limited. Its supported targets include proteins, hormones, pathogens, and other molecules. These features make the method useful for immunology studies, biomarker profiling, disease diagnostics, and drug development research.
In biology, the method links antibody specificity with multiplex measurement of molecules relevant to disease and biological responses. Researchers can apply it to immunology investigations, evaluate biomarker profiles, support disease diagnostic studies, or examine targets during drug development. Its ability to analyze several analytes while reducing sample and reagent requirements supports efficient experimental workflows.