Cotton Bollworm Colony

A cotton bollworm colony is a laboratory-maintained population of cotton bollworms, typically Helicoverpa armigera, kept through successive generations for biological research and standardized experiments. Researchers regulate environmental conditions such as temperature, humidity, light, and nutrition so eggs hatch, larvae develop, pupate, and adults reproduce in a repeatable life cycle. These colonies provide a consistent source of insects for studying development, feeding behavior, host–plant interactions, insecticide susceptibility, and pest-management strategies. By reducing variation associated with field-caught insects, colony-based experiments improve comparisons among treatments and support research on crop protection, resistance evolution, and the biology of this economically important agricultural pest.

Cotton Bollworm Colony - Related Videos

Research

JoVE Journal - Immunology and Infection

Protocols for Investigating the Host-tissue Distribution, Transmission-mode, and Effect on the Host Fitness of a Densovirus in the Cotton Bollworm

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Cited by 1 •

2017

Here, we present a protocol to investigate the host-tissue distribution, transmission mode, and effect on the host fitness of a densovirus within a lepidopteran species, the cotton bollworm. This protocol can also be used for studying the interaction between other orally-transmitted viruses and their insect hosts.

Fabricating Cotton Analytical Devices

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2016

To investigate simple fabrication approaches for multiple assay needs, we created a fluid-absorbing channel system made of cotton material. This device was used to establish a multiple detection platform, and solve contamination issues that commonly affect lateral flow-based biomedical devices, for clinical urinalysis of nitrite, total protein, and urobilinogen.

Agrobacterium-Mediated Virus-Induced Gene Silencing Assay In Cotton

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Cited by 159 •

2011

We present the detailed protocol for Agrobacterium-mediated virus-induced gene silencing (VIGS) assay in cotton. The tobacco rattle virus (TRV)-derived VIGS vectors were deployed to induce RNA silencing of cotton GrCLA1, Cloroplastos alterados 1 gene. The albino phenotype caused by silencing GrCLA1 was observed at the seedling stage within 2 weeks after inoculation.

Keratinocyte Colony Formation Assay: An In Vitro Assay to Assess the Ability of Keratinocytes to Grow into Colonies

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2023

In this video, we perform a clonogenic assay of keratinocytes harvested from adult mice to determine the ability of individual cells to proliferate and form colonies. This keratinocyte harvesting technique is useful for studying other downstream aspects of cutaneous chemical carcinogenesis and tumor promotion.

Screening Cotton Genotypes for Reniform Nematode Resistance

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2019

Here, a protocol is presented for the rapid non-destructive screening of cotton genotypes for reniform nematode resistance. The protocol involves visually examining the roots of nematode-infected cotton seedlings to determine infection response. The vegetative shoot from each plant is then propagated to recover plants for seed production.

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