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Agrobacterium-Mediated Virus-Induced Gene Silencing Assay In Cotton
 

Agrobacterium-Mediated Virus-Induced Gene Silencing Assay In Cotton

Article DOI: 10.3791/2938
August 20th, 2011

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Summary August 20th, 2011

We present the detailed protocol for Agrobacterium-mediated virus-induced gene silencing (VIGS) assay in cotton. The tobacco rattle virus (TRV)-derived VIGS vectors were deployed to induce RNA silencing of cotton GrCLA1, Cloroplastos alterados 1 gene. The albino phenotype caused by silencing GrCLA1 was observed at the seedling stage within 2 weeks after inoculation.

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