Cyanobacterial Cells

Cyanobacterial cells are photosynthetic prokaryotic cells that convert light energy into chemical energy and contribute substantially to aquatic and terrestrial ecosystems. Their thylakoid membranes contain photosynthetic pigments that drive electron transport, generate ATP and reducing power, and support carbon fixation in the cytoplasm; some species also differentiate specialized cells for nitrogen fixation. Studying cyanobacterial cells helps explain the evolution of oxygen-producing photosynthesis, microbial ecology, and global carbon and nitrogen cycling. Their metabolic versatility also supports research in biotechnology, including renewable compound production, environmental monitoring, and the development of engineered systems for sustainable biomanufacturing.

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JoVE EoE - Bacterial Growth and Techniques

Preparation of Cyanobacterial Culture for Biomass Production

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2025

Source: Jiang, H., Ho, M. Isolation and Characterization of Intact Phycobilisome in Cyanobacteria. J. Vis. Exp.(2021).This video demonstrates the procedure for culturing cyanobacteria to obtain biomass by growing cells under continuous illumination with carbon dioxide supply and nutrient-rich conditions, followed by harvesting via centrifugation.

Photobleaching Cyanobacterial Pigments for Imaging Growth-Regulating Proteins

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2026

Source: Zhan, Y., et al. Photobleaching Enables Super-resolution Imaging of the FtsZ Ring in the Cyanobacterium Prochlorococcus. J. Vis. Exp. (2018).This video demonstrates the procedure for photobleaching pigments in cyanobacterial cells using xenon light under cold conditions to improve the fluorescence imaging of growth-regulating proteins.

A Technique to Detect Cyanobacterial Strains Using an Antibody Microarray Chip

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2025

This video demonstrates a method to detect the Cyanobacterial strains in an environmental sample using an antibody microarray chip. Samples carrying various bacteria interact with printed antibodies, and immunostaining with fluorophore-labeled anti-cyanobacterial-strain antibodies reveals the presence of specific cyanobacterial strains through fluorescence in distinct spots.

Generation of Marked and Markerless Mutants in Model Cyanobacterial Species

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Cited by 36 •

2016

Introducing multiple genomic alterations into cyanobacteria is an essential tool in the development of strains for industrial and basic research purposes. We describe a system for generating unmarked mutants in the model cyanobacterial species Synechocystis sp. PCC6803 and marked mutants in Synechococcus sp. PCC7002.

Long-Term Cryopreservation of Cyanobacterial Strains Using Glycerol or Dimethyl Sulfoxide

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2025

Source: Lea-Smith, D. J., et al. Generation of Marked and Markerless Mutants in Model Cyanobacterial Species. J. Vis. Exp. (2016)This video demonstrates the cryopreservation of cyanobacterial strains by combining concentrated cell suspensions with glycerol or DMSO and storing them at low temperatures, enabling long-term maintenance of viable cultures for photosynthesis and genetic studies.

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