Draining Lymph Nodes
Draining Lymph Nodes - Related Videos
02:29
Harvesting an Inguinal Draining Lymph Node from a Murine Model
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2025
This video demonstrates the surgical isolation procedure of the inguinal draining lymph node from a mouse model. Incision of the abdominal region provides access to the inguinal lymph node, which is surgically harvested. The harvested translucent inguinal lymph node can be used for immunological studies.
02:16
A Dissection Method to Harvest Murine Popliteal Draining Lymph Nodes for Ex Vivo Imaging
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2025
This video demonstrates a dissection technique to harvest popliteal draining lymph nodes from the mouse hind legs. Lymph nodes play a critical role in bridging innate and adaptive immunity, and harvesting intact lymph nodes — with a preserved tissue structure and cellular viability — can help study local and systemic immune responses.
05:45
Identifying Regulatory T Cell Subsets from Murine Pancreatic Draining Lymph Nodes
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2025
In this video, we describe a protocol for isolating single cells from murine pancreatic draining lymph nodes (PDLNs) and the characterization of regulatory T cells (Tregs) from the single-cell population using flow cytometry. Tregs are identified based on the positive expression of the cell-surface markers CD4, CD25, and Nrp1, along with the nuclear transcription factors Foxp3 and Helios.
08:06
Lymph Node Exam
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2023
Source: Richard Glickman-Simon, MD, Assistant Professor, Department of Public Health and Community Medicine, Tufts University School of Medicine, MA The lymphatic system has two main functions: to return extracellular fluid back to the venous circulation and to expose antigenic substances to the immune system. As the collected fluid passes through lymphatic channels on its way back to the systemic circulation, it encounters multiple nodes consisting of highly concentrated clusters of...
08:01
Non-surgical Intratracheal Instillation of Mice with Analysis of Lungs and Lung Draining Lymph Nodes by Flow Cytometry
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Cited by 51 •
2011
We illustrate non-surgical delivery of test materials into the lungs of anesthetized mice via the trachea. This method permits lung exposure to bacterial and viral pathogens, cytokines, antibodies, beads, chemicals, or dyes. We further describe harvesting and processing of lungs and lung draining lymph nodes (LDLNs) for flow cytometry.
FAQs
Material arriving through afferent lymphatic vessels is exposed to macrophages and dendritic cells, which filter lymph and handle antigens for immune recognition. Dendritic-cell antigen presentation can engage lymphocytes, while activated lymphocytes help coordinate an adaptive response. This arrangement links molecular information from a particular tissue to immune activity that can be examined in that tissue’s draining node.
Because lymph flow from a defined tissue region directs extracellular fluid, soluble antigens, and antigen-presenting cells to particular nodes, activity there can be interpreted in relation to that tissue. This makes node selection important when comparing immune effects associated with infection, inflammation, or tumor-related changes in different anatomical regions.
Their defined anatomical and lymphatic relationships help researchers identify nodes associated with a particular tissue region. Mapping can therefore focus attention on locations most relevant to tissue-derived immune material and possible tumor movement. This approach connects anatomy with functional immune surveillance, helping relate experimental findings to a specific lymphatic drainage pathway.
Analysis can reveal immune activity associated with the tissue that supplies the node. Researchers may use these observations to investigate responses to infection or inflammation, follow how soluble antigens and antigen-presenting cells are handled, and examine patterns relevant to tumor spread through lymphatic pathways. The node therefore provides tissue-linked information rather than an isolated immune measurement.
A typical conceptual workflow begins by relating a tissue region to its lymphatic drainage, then considers the material carried into the node. Researchers can examine filtration by macrophages and dendritic cells, antigen presentation to lymphocytes, and subsequent lymphocyte activation. This sequence helps connect tissue conditions with the organization of an adaptive immune response.
They provide a defined site for investigating how tissue-associated material enters immune surveillance and how adaptive responses are coordinated. In experimental biology, this supports studies of infection, inflammation, and tumor dissemination, while their anatomical relationships also enable sentinel lymph node mapping. Findings can consequently combine structural information with evidence of immune activity.