Dth Induction
Dth Induction - Related Videos
09:59
Imaging Effector Memory T cells in the Ear After Induction of Adoptive DTH
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Cited by 2 •
2008
Here we demonstrate a method for inducing and recording the progress of a delayed type-hypersensitivity (DTH) reaction in the rat ear. This is followed by a demonstration of the preparation of rat ear tissue for two-photon imaging of the effector / memory T cell response.
13:26
Induction and Monitoring of Active Delayed Type Hypersensitivity (DTH) in Rats
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Cited by 18 •
2007
Delayed type hypersensitivity (DTH) is an inflammatory reaction mediated by CCR7- effector memory T lymphocytes. Here we demonstrate how to induce active DTH in Lewis rats and monitor the inflammatory response.
11:33
Inductance
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2023
Source: Yong P. Chen, PhD, Department of Physics & Astronomy, College of Science, Purdue University, West Lafayette, IN This experiment will use inductive coils to demonstrate the concept of inductor and inductance. Magnetic induction will be demonstrated using a rod magnet inserted into or extracted away from the core of a coil to induce a transient electromotive force (emf) voltage in the coil, measured by a voltmeter. This experiment will also demonstrate the mutual inductance between...
14:01
Anesthesia Induction and Maintenance
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2016
Source: Kay Stewart, RVT, RLATG, CMAR; Valerie A. Schroeder, RVT, RLATG. University of Notre Dame, IN The Guide for the Care and Use of Laboratory Animals ("The Guide") states that pain assessment and alleviation are integral components of the veterinary care of laboratory animals. The definition of anesthesia is the loss of feeling or sensation. It is a dynamic event involving changes in anesthetic depth with respect to an animal's metabolism, surgical stimulation, or variations in the...
11:18
AC Induction Motor Characterization
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2023
Source: Ali Bazzi, Department of Electrical Engineering, University of Connecticut, Storrs, CT. The objectives of this experiment are to find the equivalent circuit parameters of a three-phase induction motor using the per-phase equivalent circuit and tests similar to those used in transformer characterization. In electrical engineering, an equivalent circuit (or theoretical circuit) can be determined for a given system. The equivalent circuit retains all characteristics of the original system,...
FAQs
The delayed timing reflects the sequence of cellular events after antigen challenge. Antigen-specific memory T cells become activated, release cytokines, and promote macrophage recruitment. These coordinated signals gradually produce localized tissue inflammation, so measurements taken during the 24–72-hour period can capture the developing cellular response rather than only an early reaction.
Sensitization establishes the antigen-specific immune readiness needed for a later response, whereas the challenge phase re-exposes the system to that antigen and activates memory T cells. Keeping these phases conceptually separate helps researchers relate the later inflammatory response to prior immune priming and assess whether the reaction is directed against the intended antigen.
T cells provide antigen-specific recognition and release cytokines after activation. Those cytokine signals help recruit macrophages to the affected tissue, where their accumulation contributes to the local inflammatory changes measured in the model. Examining both lymphocyte-driven signaling and macrophage recruitment therefore gives a broader picture of cellular immune activity than assessing tissue swelling alone.
A typical workflow begins with antigen sensitization, followed by a defined antigen challenge after the immune system has been primed. Researchers then observe the localized response during the subsequent 24–72 hours and assess indicators such as swelling, tissue changes, or infiltrating immune cells. This sequence links experimental timing with the underlying cellular response.
Swelling provides a measurable indicator of the local response, while tissue changes and infiltrating immune cells add structural and cellular context. Considering these readouts together can show whether antigen exposure produced localized inflammation and antigen-specific immune activity. The combination is also useful when comparing biological or preclinical models and evaluating changes caused by treatment.
In vaccine studies, the response can help assess whether immunization promotes antigen-specific cellular immunity. In treatment research, the same experimental approach can be used to test immunomodulatory treatments by examining how swelling, tissue changes, or immune-cell infiltration are altered. These applications make DTH induction relevant for studying both immune activation and regulation in preclinical biology.