E-cadherin Downregulation
E-cadherin Downregulation - Related Videos
01:25
Structure of Cadherins
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2023
The cadherins were one of the first cell adhesion molecules discovered; the term “cadherins” is based on their calcium-dependent adhering properties. The first cadherins discovered on the epithelial, neuronal, and placental cells were named E-cadherin, P-cadherin, and N-cadherin, respectively. These classical cadherins share sequence and structural similarities. Other cadherins, including those involved in cell signaling, are grouped into non-classical cadherins. This diversity of cadherins...
01:15
Receptor Downregulation in MVBs
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2023
Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded. The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR activation may...
01:19
Cadherins in Tissue Organization
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2023
The cadherins are a superfamily of cell adhesion molecules comprising over 180 variants, with specific tissues expressing a particular combination of cadherin types. Cadherins generally exhibit homophilic binding; i.e., cadherins on one cell bind to cadherins of the same or closely related type on another cell. Thus, cells of the same type have a specific affinity to bind to each other and sort themselves into clusters to form tissues. Cell Sorting During Development Cell sorting plays an...
10:16
SorLA and CLC:CLF-1-dependent Downregulation of CNTFRα as Demonstrated by Western Blotting, Inhibition of Lysosomal Enzymes, and Immunocytochemistry
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Cited by 1 •
2017
The present protocol describes how Western blotting and immunocytochemistry combined with inhibition of lysosomal enzymes can be used to demonstrate the downregulation of Ciliary Neurotrophic Factor Receptor-α (CNTFRα), which is conveyed by the interaction between Cytokine-like Factor-1 (CLF-1) and the endocytic receptor sorLA.
04:42
Imaging of Cell Shape Alteration and Cell Movement in Drosophila Gastrulation Using DE-cadherin Reporter Transgenic Flies
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2016
Herein we describe a procedure to capture live images of Drosophila gastrulation. This has enabled us to better understand the apical constriction involved in early development and further analyze mechanisms governing cellular movements during tissue structure modification.
FAQs
Several regulatory routes can reduce E-cadherin levels, including transcriptional repression, promoter methylation, altered signaling, and microRNA activity. These mechanisms act at different points in gene regulation, so researchers can study them separately or as interacting influences. Identifying the route involved helps connect changes in E-cadherin expression with the signaling pathways that control epithelial cell adhesion and identity.
Lower E-cadherin weakens the adhesion that keeps epithelial cells organized in tightly connected layers. As cell-cell attachment declines, tissue organization can change and cell motility can increase. This shift is relevant to epithelial-to-mesenchymal transition, a process associated with altered epithelial identity, and provides a mechanistic link between adhesion changes, developmental remodeling, and tumor invasion.
Promoter methylation is important because it represents a regulatory change that can reduce E-cadherin expression at the gene-control level. Examining this mechanism allows researchers to relate altered expression to molecular regulation rather than viewing adhesion loss only as a structural change. It can therefore help clarify how regulatory pathways influence epithelial identity and tissue organization.
The same broad change in cell adhesion can have different biological contexts. During development, altered E-cadherin levels may participate in tissue remodeling, whereas in cancer biology they are studied in relation to epithelial identity, increased motility, and tumor invasion. Comparing these contexts helps researchers distinguish regulated tissue reorganization from changes associated with cancer progression.
Measuring E-cadherin expression provides an indicator of changes in epithelial cell adhesion and identity. Reduced expression can direct attention toward altered tissue organization, increased motility, or epithelial-to-mesenchymal transition. When considered with the relevant regulatory mechanisms, the measurement helps researchers investigate how cell-cell adhesion changes during developmental remodeling or cancer progression.
This topic supports questions about how cells maintain epithelial organization, how regulatory pathways alter adhesion, and how changes in cell behavior contribute to tissue remodeling or tumor invasion. Researchers can also examine whether transcriptional repression, promoter methylation, altered signaling, or microRNA activity accompanies reduced expression. These questions connect molecular regulation with observable changes in tissue structure and cell movement.