Embryonic Chicken Model
Embryonic Chicken Model - Related Videos
06:56
Influenza Virus Propagation in Embryonated Chicken Eggs
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Cited by 105 •
2015
Fertile chicken eggs are widely used to produce large amounts of human influenza A virus as they provide a convenient and cost-effective system to prove high yields of virus.
08:09
A Novel Ex Ovo Banding Technique to Alter Intracardiac Hemodynamics in an Embryonic Chicken System
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Cited by 2 •
2016
For the first time we present here a reproducible banding procedure to alter hemodynamics in the developing heart ex ovo. This is achieved by partially constricting the outflow tract (OFT).
05:02
Purification of Virus from the Allantoic Fluid of Embryonated Chicken Eggs
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2026
Source: Yates, J. G. E., et al. Production of High-Titer Recombinant Newcastle Disease Virus from Allantoic Fluid. J. Vis. Exp. (2022)The video demonstrates the purification and concentration of virus particles from clarified allantoic fluid collected from virus-infected embryonated chicken eggs. It showcases a series of filtration and separation steps, including depth filtration, tangential flow filtration, and density gradient ultracentrifugation to isolate intact viruses. The virus-rich...
09:23
Parasite Induced Genetically Driven Autoimmune Chagas Heart Disease in the Chicken Model
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Cited by 12 •
2012
The inoculation of Trypanosoma cruzi in fertile eggs prior to incubation renders the parasite kDNA minicircle integration in embryo cells genome. Crossbreeding reveals the vertical transfer of the mutations to progeny. The kDNA integrates into coding regions at several chromosomes and the chickens die with an inflammatory autoimmune heart disease.
03:26
Chicken Chorioallantoic Membrane-based Cancer Modeling: An In Ovo Model to Study Cancer Cell Tumorigenesis and Metastasis
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2023
In this video, we describe the implantation of cancer cells onto a chicken chorioallantoic membrane to generate an in ovo model. The model successfully engrafts the ovarian cancer cells to study the progression of gynecological cancer.
FAQs
The embryo allows researchers to connect signaling activity with visible changes in cell differentiation, migration, and tissue shape. By observing development in vivo and manipulating embryonic structures, investigators can examine how local signals influence morphogenesis, the process by which tissues acquire organized forms. This links molecular mechanisms to whole-embryo outcomes rather than studying isolated cells alone.
Developmental stage determines which tissues, structures, and morphogenetic events are present and experimentally accessible. Studying embryos at defined stages lets researchers compare normal progression with outcomes after manipulation, imaging, or tissue grafting. This timing is especially relevant for analyzing neural patterning, limb formation, vascular development, and other processes whose features change as the embryo develops.
Tissue grafting and microinjection provide complementary ways to test developmental mechanisms. Grafting changes the relationship between embryonic tissues, while microinjection introduces an intervention into a developing system. Observing the resulting effects helps researchers determine how tissues communicate and how signaling influences differentiation, migration, or morphogenesis. These approaches turn developmental observations into experimentally testable relationships.
The system offers a practical bridge between cellular mechanisms and whole-organism development. Its accessible egg supports observation and manipulation of living embryonic tissues while reducing some of the complexity and cost associated with mammalian experiments. Consequently, researchers can investigate developmental processes in an intact vertebrate context before considering questions that require more complex animal models.
A typical workflow begins with incubating fertilized eggs until the desired developmental stage. Researchers can then create a window in the eggshell to access the embryo, perform procedures such as microinjection or tissue grafting, and maintain the preparation for observation. Imaging at defined stages records how the intervention affects tissue development, organization, or morphogenesis.
Imaging is most informative when observations are tied to defined developmental stages. It can document tissue position, structural changes, and the progression of morphogenesis while the embryo remains in vivo. This makes imaging useful for following neural patterning, limb formation, and vascular development, as well as for comparing manipulated embryos with their developmental outcomes.
Applications extend across embryology, organ formation, genetics, and disease research. Investigators can examine neural patterning, limb formation, vascular development, toxicology, and regenerative biology using observation and embryonic manipulation. The model is valuable when a study needs both cellular-level interpretation and evidence of how developmental changes affect organized tissues within a vertebrate embryo.