Endophytic Microorganisms
Endophytic Microorganisms - Related Videos
15:14
Establishing Fungal Entomopathogens as Endophytes: Towards Endophytic Biological Control
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Cited by 197 •
2013
This protocol demonstrates two inoculation methods to introduce the fungal entomopathogen Beauveria bassiana as an endophyte in the common bean (Phaseolus vulgaris), in preparation for subsequent evaluations of endophytic biological control.
01:22
Root-Mediated Endophytic Colonization of Beneficial Bacteria in Rice
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2025
Begin with rice seedlings pre-germinated under dark, aseptic conditions to ensure uniform sprouting.Transfer these seedlings to a flask containing a nutrient-rich, semi-solid medium pre-inoculated with a test bacterium.As the plant grows, the roots release chemotactic compounds into the surrounding medium.These compounds stimulate bacterial proliferation, leading to adhesion, multiplication, and colony formation on the root surface.As the roots develop, the bacteria enter the plant through...
01:32
Enhancing Salt Stress Tolerance in Tomato Plants Through Endophytic Bacterial Colonization
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2025
Endophytic bacteria colonize plant tissues and promote plant growth.To study their impact on tomato plant growth under salt stress, start with sterile seedlings showing developing leaves and roots.Immerse the roots of the experimental seedling in a suspension of endophytic bacteria; leave the control uninoculated.Transfer both groups into sterilized soil to prevent external microbial interference and incubate in a growth chamber.From the soil, the bacteria enter through transient root openings,...
09:57
Quantifying Environmental Microorganisms and Viruses Using qPCR
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2023
Source: Laboratories of Dr. Ian Pepper and Dr. Charles Gerba - The University of Arizona Demonstrating Author: Bradley Schmitz Quantitative polymerase chain reaction (qPCR), also known as real-time PCR, is a widely-used molecular technique for enumerating microorganisms in the environment. Prior to this approach, quantifying microorganisms was limited largely to classical culture-based techniques. However, the culturing of microbes from environmental samples can be particularly challenging, and...
01:29
Types of Microorganisms
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2025
Microorganisms are a diverse group of microscopic entities broadly categorized into cellular and acellular types based on their structural organization. Cellular microorganisms include bacteria, archaea, fungi, protozoa, and algae, while acellular microorganisms are represented by viruses.Cellular MicroorganismsBacteriaBacteria, tiny prokaryotic organisms, exhibit fascinating shapes such as rods, spheres, and spirals. They adapt to diverse habitats, including soil, water, and human-associated...
FAQs
Entry can occur through natural openings, wounds, or developing tissues. After entering, the microorganisms establish internal associations rather than causing apparent disease in healthy tissue. This location allows microbial activities to interact closely with plant cells and signaling processes, creating opportunities for effects on nutrient acquisition, hormone balance, defense responses, growth, and tolerance to environmental stress.
Microbial metabolites can alter how a plant acquires nutrients, regulates hormones, and activates defense responses. These effects arise through chemical and biological interactions between the resident microorganism and plant tissues. Consequently, endophytic associations may influence plant growth and stress tolerance, while also affecting how effectively the plant responds to potential pathogens.
Plant signaling helps coordinate the effects of internal microorganisms on physiology and defense. Signals from the plant can interact with microbial activities, including metabolite production, to influence hormone balance and protective responses. Studying these exchanges helps biology researchers explain why particular associations may support growth, improve stress tolerance, or contribute to resistance against pathogens.
Researchers examine endophytes in relation to plant growth, stress tolerance, pathogen resistance, nutrient acquisition, hormone balance, and defense responses. They also consider how the associations function within plant biology and ecology. This broad approach connects microbial activity with observable plant outcomes and clarifies the ecological significance of microorganisms living inside healthy tissues.
Their effects on plant growth, stress tolerance, and pathogen resistance make endophytic microorganisms relevant to sustainable agriculture. They may also support biological control, in which beneficial biological activity contributes to limiting harmful organisms. These applications reflect the possibility of using plant–microbe associations to support crop performance while drawing on naturally occurring biological processes.
Endophytic bacteria, fungi, and other microbes can produce bioactive compounds, meaning substances with biological effects. These compounds make endophytes valuable subjects for natural-product discovery and may also contribute to biological control. Studying their metabolites connects plant-associated microbial ecology with efforts to identify useful compounds for agricultural and other biological applications.