Ethylene Glycol
Ethylene Glycol - Related Videos
06:00
Cryopreservation of Mouse Embryos by Ethylene Glycol-Based Vitrification
0 Views •
Cited by 17 •
2011
An ethylene glycol-based vitrification method for mouse embryos is described. It is advantageous to other methods in its simplicity and low embryonic toxicity, and therefore can be broadly applicable to many strains of mice, including inbred and gene-modified mice.
15:33
Microwave-assisted Functionalization of Poly(ethylene glycol) and On-resin Peptides for Use in Chain Polymerizations and Hydrogel Formation
0 Views •
Cited by 32 •
2013
This video will illustrate a rapid, efficient method to methacrylate poly(ethylene glycol), enabling chain polymerizations and hydrogel synthesis. It will demonstrate how to similarly introduce methacrylamide functionalities into peptides, detail common analytical methods to assess functionalization efficiency, provide suggestions for troubleshooting and advanced modifications, and demonstrate typical hydrogel characterization techniques.
04:20
Investigating the Bacterial Response to Ethylene Using an Ethylene-Releasing Compound
0 Views •
2025
Source: Augimeri, R. V. et. al., Utilizing the Ethylene-releasing Compound, 2-Chloroethylphosphonic Acid, as a Tool to Study Ethylene Response in Bacteria. J. Vis. Exp. (2016)This video demonstrates the effect of ethylene generated by 2-chloroethylphosphonic acid (CEPA) on bacterial growth and cellulose production. Ethylene activates signaling pathways that stimulate cellulose synthesis, resulting in enhanced pellicle formation. These results highlight the bacterial response to ethylene.
08:51
Utilizing the Ethylene-releasing Compound, 2-Chloroethylphosphonic Acid, as a Tool to Study Ethylene Response in Bacteria
0 Views •
Cited by 1 •
2016
The protocols outlined herein facilitate the convenient investigation of bacterial ethylene responses by utilizing 2-chloroethylphosphonic acid (CEPA). Ethylene is produced in situ through the decomposition of CEPA in an aqueous bacterial growth medium, circumventing the requirement for pure ethylene gas.
08:56
Catalytic Reactor: Hydrogenation of Ethylene
0 Views •
2023
Source: Kerry M. Dooley and Michael G. Benton, Department of Chemical Engineering, Louisiana State University, Baton Rouge, LA The hydrogenation of ethylene (C2H4) to ethane (C2H6) has often been studied as a model reduction reaction in characterizing new metal catalysts.1-2 While supported nickel is not the most active metal catalyst for this reaction, it is active enough that reaction can take place at < 200°C. The reaction typically involves adsorbed, dissociated hydrogen (H2) reacting...
FAQs
Its metabolism proceeds through a sequence of oxidation products rather than stopping at the parent compound. Alcohol dehydrogenase first converts ethylene glycol to glycolaldehyde, followed by formation of glycolic acid, glyoxylic acid, and finally oxalic acid. This progression is important because the later metabolites, rather than ethylene glycol alone, are associated with severe metabolic disruption and kidney injury.
The metabolites generated during ethylene glycol breakdown include glycolic, glyoxylic, and oxalic acids. Their accumulation contributes to severe metabolic acidosis, a condition in which body fluids become abnormally acidic. This biochemical outcome makes the metabolic pathway central to poisoning research, because toxicity depends on enzymatic conversion and metabolite formation rather than simply on exposure to the original compound.
Oxalic acid can generate calcium oxalate crystals in biological systems. These crystals are associated with kidney damage, linking the final stage of ethylene glycol metabolism to a physical source of tissue injury. Studying this relationship helps explain why renal effects are a major concern in ethylene glycol poisoning and why metabolite formation is important when interpreting biological outcomes.
Ethylene glycol has contrasting biological roles that depend strongly on how it is used and at what concentration. Controlled concentrations can lower the freezing point of aqueous solutions and limit ice formation, supporting preservation. In poisoning contexts, metabolism produces acidic compounds and calcium oxalate, so uncontrolled exposure can instead lead to severe metabolic acidosis and kidney damage.
In cryobiology, ethylene glycol functions as a laboratory cryoprotectant for cells and tissues. By lowering the freezing point of aqueous solutions, it helps reduce ice formation during preservation. This application focuses on controlling the physical behavior of water around biological material, allowing researchers to investigate preservation conditions while distinguishing that purpose from the compound’s toxic metabolic effects.
Research commonly follows both biochemical and tissue-level outcomes. Biochemically, investigators examine conversion through glycolaldehyde, glycolic acid, glyoxylic acid, and oxalic acid, along with the resulting metabolic acidosis. At the tissue level, they assess calcium oxalate crystal formation and kidney damage. Together, these outcomes connect enzyme activity, metabolite accumulation, and organ injury.