Exopolysaccharide

Exopolysaccharides are carbohydrate polymers produced and released by microorganisms, where they form extracellular layers that influence interactions between cells and their environment. Enzymes assemble repeating sugar units through glycosyltransferase-driven pathways, and the resulting polymers are exported to create hydrated matrices that support cell adhesion, biofilm formation, and protection from environmental stresses. In biology, exopolysaccharides help explain microbial colonization, cooperation, and persistence on living or nonliving surfaces. Their roles in surface attachment and matrix formation also make them relevant to biotechnology, including biomaterials, food production, and the study of microbial communities and host-microbe interactions.

Exopolysaccharide - Related Videos

Research

JoVE EoE - Bacterial Pathogenesis and Host Interactions

Investigating the Human Gut Microbial Activity on Exopolysaccharides

0 Views •

2026

Source: Hu, Y. et.al., Analysis of Interactions between Endobiotics and Human Gut Microbiota Using In Vitro Bath Fermentation Systems. J. Vis. Exp. (2019)This video demonstrates the analysis of short-chain fatty acid production by human gut microbiota in response to exopolysaccharides. This highlights microbial fermentation products separation and quantification using gas chromatography. The results reveal that exopolysaccharides modulate microbial metabolites that influence host-pathogen...

Performing Thin-Layer Chromatography to Assess Bacterial Exopolysaccharide Degradation

0 Views •

2026

Source: Hu, Y., et al. Analysis of Interactions between Endobiotics and Human Gut Microbiota Using In Vitro Bath Fermentation Systems. J. Vis. Exp. (2019)This video demonstrates the use of thin-layer chromatography (TLC) to assess the degradation of exopolysaccharides (EPS) by human fecal microbiota. EPS fragments in fermentation supernatants are separated based on size and polarity using TLC, then visualized as colored bands following carbohydrate-specific staining.

Research

JoVE Journal - Biology
Free Sample

Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis

0 Views •

Cited by 18 •

2016

We present an automated modular high-throughput-method for the identification and characterization of microbial exopolysaccharides in small scale. This method combines a fast preselection to analyze the total amount of secreted polysaccharides with a detailed carbohydrate fingerprint to enable the fast screening of newly isolated bacterial strains or entire strain collections.

Visualization of Pseudomonas aeruginosa in Sputum Samples from Cystic Fibrosis Patients

0 Views •

2026

Source: Jackson, L., et al. Visualization of Pseudomonas aeruginosa within the Sputum of Cystic Fibrosis Patients. J. Vis. Exp. (2020)This video demonstrates the in situ visualization of Pseudomonas aeruginosa within chemically cleared cystic fibrosis sputum using fluorescent labeling and confocal microscopy to reveal bacterial localization and biofilm structure.

Assessing Bacterial Respiration in Response to Silver Nanoparticles

0 Views •

2025

Begin with a slurry containing bacterial cells treated with silver nanoparticles suspended in an exopolysaccharide matrix.Add a phosphate buffer to the slurry to maintain a stable pH.Next, introduce a solution containing glucose and a soluble redox indicator, then incubate the mixture. The redox indicator acts as an electron acceptor.During incubation, glucose and the redox indicator enter metabolically active bacterial cells.The cells utilize glucose during respiration, generating...

View All Results

FAQs

Related Topics