Fibered Confocal Endoscopy
Fibered Confocal Endoscopy - Related Videos
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This method visualizes the native structure of collagen fibers in the vitreous body using confocal reflectance microscopy.
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Video-rate Scanning Confocal Microscopy and Microendoscopy
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The complete construction of a custom, real-time confocal scanning imaging system is described. This system, which can be readily used for video-rate microscopy and microendoscopy, allows for an array of imaging geometries and applications not accessible using standard commercial confocal systems, at a fraction of the cost.
07:07
Endoscopy Guided Photoablation of Endometrial Cysts using a 980 nm Laser with a Contact Fiber in Mares
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The aim of this report is to describe the photoablation of endometrial cysts in the mare using an endoscopic laser device, and to discuss the interest and limitations of this new approach to deal with low fertility issues.
07:54
Drug-Induced Sleep Endoscopy (DISE) with Target Controlled Infusion (TCI) and Bispectral Analysis in Obstructive Sleep Apnea
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The aim of this study was to establish a standardized protocol for sleep endoscopy to differentiate obstruction patterns in obstructive sleep apnea (OSA). Target-controlled infusion (TCI) of the sedative was combined with real-time monitoring of the depth of sedation using bispectral analysis.
08:28
Microfluidic Dry-spinning and Characterization of Regenerated Silk Fibroin Fibers
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A protocol for the microfluidic spinning and microstructure characterization of regenerated silk fibroin monofilament is presented.
FAQs
The coherent fiber bundle preserves the spatial arrangement of light-carrying fibers between the probe and imaging system. Laser illumination travels through these fibers to the tissue, and fluorescence emitted from the tissue returns through the same bundle. This arrangement allows the system to obtain cellular-scale views through a flexible probe while maintaining access to living tissue.
Confocal detection rejects light originating outside the focal plane, reducing out-of-focus signal in the collected image. The resulting optical sectioning helps distinguish cellular structures and tissue organization more clearly than an image formed from mixed-depth fluorescence. This improves interpretation of biological features within intact organs and supports high-resolution examination during endoscopic access.
Fluorescence provides the emitted signal used to form the image after laser illumination reaches the tissue. Because the returning fluorescence travels through the same fiber bundle, the system can collect information from the illuminated region without removing a specimen. The signal therefore supports direct examination of cellular structures and biological organization in living tissue.
Rather than requiring tissue removal for cellular examination, fibered confocal endoscopy obtains images from living tissue through an endoscopically accessible site. This distinction preserves the observed tissue in its biological setting and enables visualization during ongoing processes. It is especially relevant when researchers need to assess intact organs or follow changes over time.
A flexible fiber-optic probe is positioned for access to the tissue, and laser light is transmitted through its coherent fiber bundle. Tissue fluorescence then returns through the same fibers to a confocal detection system, which removes out-of-focus light and produces an optically sectioned image. The sequence provides immediate views of the examined biological region.
Biologists may choose fibered confocal endoscopy when they need real-time cellular or tissue-level information from an intact organ rather than an excised specimen. Its portability and compatibility with endoscopic access also support longitudinal studies, in which biological changes are assessed repeatedly, and targeted assessment of particular regions within living tissue.
Images generated with this approach can reveal cellular structures, patterns of tissue organization, and dynamic biological processes in living tissue. These complementary levels of information help researchers connect individual cell appearance with the broader arrangement of an intact organ. Real-time acquisition is useful when the biological state may change during observation.