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Chapters
- 00:05Title
- 01:01Setting Up the Resonant Galvanometric Mirror and Relay Optics
- 04:56Setting Up the Second Scanning Mirror and Rotating the Microscope
- 06:37Setting Up the Scan, Tube, and Objective Lenses
- 08:17Setting Up and Aligning the Confocal Pinhole and Detector
- 12:38The Assembled System and Representative Images
- 13:43Conclusion
The complete construction of a custom, real-time confocal scanning imaging system is described. This system, which can be readily used for video-rate microscopy and microendoscopy, allows for an array of imaging geometries and applications not accessible using standard commercial confocal systems, at a fraction of the cost.
