Fibroblast Epithelial Interaction
Fibroblast Epithelial Interaction - Related Videos
15:43
Experimental Generation of Carcinoma-Associated Fibroblasts (CAFs) from Human Mammary Fibroblasts
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Cited by 25 •
2011
Carcinoma-associated fibroblasts (CAFs) rich in myofibroblasts present within the tumour stroma, play a major role in driving tumour progression. We developed a coimplantation tumour xengraft model for experimentally generating CAFs from human mammary fibroblasts. The protocol describes how to establish CAF myofibroblasts that acquire an ability to promote tumourigenesis.
12:42
Heterotypic Three-dimensional In Vitro Modeling of Stromal-Epithelial Interactions During Ovarian Cancer Initiation and Progression
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Cited by 20 •
2012
We describe methodologies for establishing in vitro heterotypic three-dimensional models comprising ovarian fibroblasts and normal ovarian surface or ovarian cancer epithelial cells. We discuss the use of these models to study stromal-epithelial interactions that occur during ovarian cancer development.
05:05
Tumor Cell Invasion Assay: A 3D In Vitro Native Matrix-based Method to Assess Epithelial-mesenchymal Cell Interactions
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2023
This video describes the technique of utilizing a native matrix to study tumor cell invasion. The technique used in this assay provides the ability to assess epithelial-mesenchymal cell interactions in a 3D setting without the need for a synthetic or foreign matrix.
01:20
FimH-Mediated Host Interaction of Enterobacter cloacae in a Bladder Epithelial Cell Model
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2026
Take cultures of human urinary bladder epithelial cells in a multiwell plate.Incubate the control culture with wild-type Enterobacter cloacae, a pathogenic bacterium with pili, hair-like structures on the bacterial surface.The FimH subunit of the pili binds to the host cell receptor, anchoring the wild-type bacteria to the host cell.Incubate the experimental culture with a mutant Enterobacter cloacae lacking FimH.Without FimH, the mutant bacteria fail to adhere to the host...
12:25
Microfluidic Co-culture of Epithelial Cells and Bacteria for Investigating Soluble Signal-mediated Interactions
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Cited by 17 •
2010
This protocol describes a microfluidic co-culture model for simultaneous and localized culture of epithelial cells and bacteria. This model can be used for investigating the role of different soluble molecular signals on pathogenesis as well as screen the effectiveness of putative probiotic bacterial strains.
FAQs
Fibroblast–epithelial interaction coordinates tissue behavior through two complementary routes: fibroblasts remodel the extracellular matrix and release paracrine signals, while epithelial cells produce factors that alter fibroblast activity. Because each population can modify the other, communication links matrix organization with epithelial responses, helping coordinate epithelial closure and the accompanying stromal remodeling during repair.
Extracellular matrix remodeling provides a structural component of the interaction, alongside soluble paracrine communication. Fibroblast activity can therefore influence how epithelial cells behave within their surrounding tissue environment, while epithelial signals regulate fibroblast matrix production. This connection helps explain how changes in stromal organization can affect tissue repair and barrier-related epithelial functions.
Dysregulated communication can shift a coordinated repair response toward disease-associated tissue changes. The source context identifies fibrosis and tumor development as settings in which fibroblast–epithelial signaling may become abnormal. Studying these conditions helps distinguish mechanisms that support epithelial closure and stromal remodeling from interactions associated with persistent or harmful tissue organization.
A co-culture system brings fibroblasts and epithelial cells together so investigators can examine reciprocal effects between the two populations. Researchers can assess epithelial closure, fibroblast migration, stromal remodeling, and matrix production within the same experimental model. This approach makes it possible to study communication in a controlled setting while preserving the interaction between connective-tissue and epithelial components.
Tissue models can show how communication changes tissue organization, repair, and barrier function in a more integrated context than observations of either cell type alone. Relevant outcomes include epithelial closure, stromal remodeling, fibroblast migration, and matrix production. Comparing these responses can clarify whether signaling supports normal repair or resembles changes associated with fibrosis or tumor development.
In biology, fibroblast–epithelial interaction provides a framework for linking connective-tissue behavior with epithelial repair and barrier maintenance. In regenerative medicine, understanding these reciprocal signals may help researchers design strategies that support coordinated tissue restoration. The same models also provide context for investigating disease mechanisms when communication contributes to fibrosis or tumor development.