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Chapters
- 00:00Title
- 00:42Introduction
- 01:29Preparation of Replica Molds from Master Silicone Wafer
- 04:00Bonding, Assemebling and Preparing Multilayer PDMS Devices
- 06:55Cultivation of Commensal E.coli Biofilm
- 08:05Introduction of Eukaryotic Cells Around Commensal Bacteria
- 10:55Modeling the Gut Bacterial Infection Environment Using a Tri-Layer Microfluidic Device
- 11:40Conclusion
This protocol describes a microfluidic co-culture model for simultaneous and localized culture of epithelial cells and bacteria. This model can be used for investigating the role of different soluble molecular signals on pathogenesis as well as screen the effectiveness of putative probiotic bacterial strains.
