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Microfluidic Co-culture of Epithelial Cells and Bacteria for Investigating Soluble Signal-mediated Interactions

Article DOI: 10.3791/1749-v • 12:25 min • April 20th, 2010
April 20th, 2010 •
1, 1, 1,2
1McFerrin Department of Chemical Engineering, Texas A&M University, 2Department of Biomedical Engineering, Texas A&M University

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This protocol describes a microfluidic co-culture model for simultaneous and localized culture of epithelial cells and bacteria. This model can be used for investigating the role of different soluble molecular signals on pathogenesis as well as screen the effectiveness of putative probiotic bacterial strains.

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Microfluidic Co-culture Epithelial Cells Bacteria Soluble Signals Interactions Gastrointestinal Tract Commensal Bacteria Pathogen Colonization Culture Methods Microenvironment Microfluidic Co-culture Model Eukaryotic Cells Escherichia Coli Biofilm Enterohemorrhagic E. Coli (EHEC) GI Tract Infection
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