Fish Technique
Fish Technique - Related Videos
03:54
Zebrafish Brain Dissection: A Technique of Fish Neurobiology
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2023
This video describes the technique of microdissection of the brain from an adult zebrafish. This method helps in obtaining whole brain derived neuorospheres that can be studied further.
07:25
Basic Methods for the Study of Reproductive Ecology of Fish in Aquaria
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Cited by 2 •
2017
A series of basic methods to enable the study of the reproductive ecology of fish kept in aquaria are described. These are useful protocols for collecting fish using SCUBA, transporting live fish, and observing the reproductive behavior of wild-caught fish kept in aquaria.
05:29
3D DNA FISH: A Technique to Locate a Specific Gene on a Chromosome
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2023
Circulating tumor cells (CTC) isolation and characterization from the blood of cancer patients is a type of non-invasive liquid biopsy and associated with relapse and disease progression. This video describes the technique of DNA fluorescence-in-situ-hybridization (DNA FISH) on CTCs captured on a medical wire, to study a specific gene of interest.
07:41
Balloon Tag Manufacturing Technique for Sensor Fish and Live Fish Recovery
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Cited by 2 •
2023
A protocol is presented for designing and manufacturing balloon tags to recover Sensor Fish and live fish, allowing assessment of their physical condition and biological performance in hydraulic structures. The method optimizes balloon tag performance by considering factors such as balloon volume, inflation/deflation times, component selection, and the characteristics of the injected water.
02:32
Osmoregulation in Fishes
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2019
When cells are placed in a hypotonic (low-salt) fluid, they can swell and burst. Meanwhile, cells in a hypertonic solution—with a higher salt concentration—can shrivel and die. How do fish cells avoid these gruesome fates in hypotonic freshwater or hypertonic seawater environments? Fish employ osmoregulatory strategies to balance bodily levels of water and dissolved ions (i.e., solutes), such as sodium and chloride. Imagine two solutions separated by a membrane that is permeable to water.
FAQs
Specificity comes from pairing each fluorescently labeled nucleic acid probe with a complementary DNA or RNA target. Under controlled hybridization conditions, matching sequences associate more effectively than nonmatching sequences, allowing fluorescence microscopy to distinguish the intended genomic or transcript target. Probe choice and hybridization control therefore determine how reliably researchers can locate a sequence within cells, chromosomes, or tissue sections.
Keeping cells, chromosomes, or tissue sections intact preserves the spatial position of the sequences being examined. This allows researchers to relate a fluorescent signal to a particular chromosome, cellular region, or tissue context rather than measuring nucleic acids after extraction. That spatial information supports studies of genome organization, development, gene location, and disease-associated cellular changes.
Signal number and distribution can provide information about the presence, location, and relative abundance of targeted DNA or RNA sequences. Researchers can examine whether signals occur at expected chromosomal or cellular sites and identify altered patterns across samples. These observations help support chromosome analysis, gene mapping, detection of numerical or structural abnormalities, and investigation of gene expression patterns.
The distinguishing feature is the retention of spatial context. Rather than reporting only whether a target sequence is present in a prepared sample, FISH shows where that sequence occurs within intact cells, chromosomes, or tissue sections. This makes the technique especially useful when location is biologically meaningful, including investigations of genome organization, developmental processes, tissue patterns, and disease.
A typical workflow places cells, chromosomes, or tissue sections on a suitable preparation, exposes them to fluorescently labeled probes, and allows hybridization under controlled conditions. After probe binding, fluorescence microscopy is used to visualize the resulting signals and assess their location or abundance. The specific interpretation depends on whether the target is DNA or RNA and on the biological question.
The essential components are a biological preparation containing the target, a fluorescently labeled nucleic acid probe, controlled hybridization conditions, and fluorescence microscopy. Hybridization conditions matter because probes must interact with complementary sequences sufficiently for the intended signal to be observed. Maintaining the relevant cellular, chromosomal, or tissue structure also supports meaningful spatial interpretation of the fluorescence pattern.
FISH is useful when researchers need to connect a specific DNA or RNA sequence with its cellular or chromosomal location. Applications include gene mapping, chromosome analysis, detection of numerical or structural abnormalities, pathogen detection, and examination of gene expression patterns. Its spatial readout also contributes to research on genome organization, development, and disease-related changes.