Flagella

Flagella are whip-like cellular appendages that generate movement or help position cells in fluid environments, making them important across microbiology and cell biology. In bacteria, a flagellum rotates like a propeller when ion flow through a membrane-embedded motor drives its basal body, hook, and filament; in eukaryotic cells, coordinated microtubule sliding within the axoneme produces bending waves. These structures support bacterial chemotaxis, allowing cells to move toward favorable conditions and away from harmful ones, while eukaryotic flagella propel sperm and move fluid across tissues. Studying flagellar assembly, motility, and regulation clarifies cell behavior, infection mechanisms, and evolutionary relationships.

Flagella - Related Videos

Education

JoVE Core - Microbiology

Flagella and Motility in Bacteria

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2025

Flagella are specialized, thread-like structures that extend from a bacteria's cell envelope. They play a crucial role in motility and chemotaxis. Their structural organization and functioning exemplify sophisticated biological engineering, enabling bacterial survival and adaptability in diverse environments.Structure of the FlagellumA bacterial flagellum consists of three key components: the filament, the hook, and basal body. The filament, a long, helical structure composed of repeating...

Research

JoVE Journal - Biology

Investigating Flagella-Driven Motility in Escherichia coli by Applying Three Established Techniques in a Series

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Cited by 17 •

2020

Many bacteria use flagella-driven motility to navigate their environment and colonize favorable surroundings both individually and as a collective. Demonstrated here is the use of three established methods that exploit motility as a selection tool to identify components/pathways contributing to swimming and swarming motility.

Visualization of Bacterial Motility Using Redox Dye–Supplemented Semisolid Medium

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2025

Source: Chu, W., and Zhuang, X. Visualizing Bacterial Motility Based on a Color Reaction. J. Vis. Exp. (2022)This video demonstrates a redox dye-based semisolid agar assay for assessing bacterial motility by visually distinguishing motile and non-motile strains.

Visualizing Single Molecular Complexes In Vivo Using Advanced Fluorescence Microscopy

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Cited by 8 •

2009

Here we demonstrate the protocols for performing single-molecule fluorescence microscopy on living bacterial cells to enable functional molecular complexes to be detected, tracked and quantified.

Microdissection of Black Widow Spider Silk-producing Glands

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Cited by 18 •

2011

Here we describe an efficient strategy to remove the silk-producing glands from the abdomen of female black widow spiders. This procedure allows the rapid isolation of the seven distinct silk-producing glands in a highly purified fashion, an important process for investigators studying spider silk production and fiber assembly.

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