Fly Head Dissection

Fly head dissection is a microsurgical method for exposing and examining the anatomical structures of a fly’s head, making it useful for studying insect biology at cellular and tissue scales. Under a stereomicroscope, the specimen is immobilized and the head capsule is carefully opened with fine instruments so that tissues such as the brain, eyes, and mouthparts can be isolated or viewed while minimizing damage. This preparation supports anatomical observation, histological staining, immunolabeling, and targeted analysis of neural or sensory structures. In biology education and research, it helps connect head anatomy with behavior, sensory processing, development, and nervous-system function.

Fly Head Dissection - Related Videos

Research

JoVE EoE - Drosophila melanogaster (fruit fly)

Drosophila Adult Brain Dissection: A Method in Fly Neurobiology

0 Views •

2023

This video describes how to dissect and isolate the adult Drosophila brain–a procedure necessary for visualizing the organ, which is otherwise obscured by the cuticle, eye, and tracheal tissue. The example protocol shows a detailed demonstration yielding high-quality preparations that can be used for immunostaining and imaging methods in Drosophila neurobiology.

Dissection of the Midgut from an Adult Fly: A Method for Isolating the Digestive Tract

0 Views •

2023

In this video, we highlight a dissection of the adult Drosophila intestine and collection of midguts suitable for single-cell dissociation and FACS in order to isolate intestinal stem cells.

A Technique to Inflict Closed-Head Traumatic Brain Injury in Drosophila Flies

0 Views •

2025

This video demonstrates a procedure to inflict a closed-head traumatic injury in anesthetized Drosophila flies using a strike device with a gas-propelled impactor. The injury affects neuronal function and impairs walking ability.

Dissection and Mounting of Fruit Fly Larval Brain Explants

0 Views •

2025

This video demonstrates the dissection and mounting of brain explants from Drosophila larvae. The central nervous system (CNS), attached to eye disks, is extracted from the larvae and then mounted on a glass slide in a physiological saline solution.

Malpighian Tubule Dissection from an Adult Fly: Isolating an Osmoregulatory and Excretory Organ

0 Views •

2023

This article describes the dissection of the anterior Malpighian tubules (MT) from a female Drosophila fly and an example protocol from Rossano et al. (2017) in which the MTs are prepared for live imaging in a perfusion chamber.

View All Results

FAQs

Related Topics