Fusion Pcr
Fusion Pcr - Related Videos
02:45
Nuclear Fusion
0 Views •
2020
The process of converting very light nuclei into heavier nuclei is also accompanied by the conversion of mass into large amounts of energy, a process called fusion. The principal source of energy in the sun is a net fusion reaction in which four hydrogen nuclei fuse and ultimately produce one helium nucleus and two positrons. A helium nucleus has a mass that is 0.7% less than that of four hydrogen nuclei; this lost mass is converted into energy during the fusion. This reaction produces about...
09:27
Generation of Fluorescent Protein Fusions in Candida Species
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Cited by 6 •
2017
PCR-mediated gene modification can be used to generate fluorescent protein fusions in Candida species, which facilitates visualization and quantitation of yeast cells and proteins. Herein, we present a strategy for constructing a fluorescent protein fusion (Eno1-FP) in Candida parapsilosis.
09:19
Analysis of SNARE-mediated Membrane Fusion Using an Enzymatic Cell Fusion Assay
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2012
We have developed a cell fusion assay that quantifies SNARE-mediated membrane fusion events by activated expression of β-galactosidase.
08:51
Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR (RS-PCR)
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Cited by 9 •
2016
Here, ribosomal spacer PCR (RS-PCR) is used together with a miniaturized electrophoresis system as a fast and high resolution method for genotyping S. aureus at moderate costs allowing a high throughput.
03:41
Agarose Gel Electrophoresis of DNA Amplicons Post PCR: A Method to Analyze Products of Multiplex PCR and Evaluate PCR Reaction Success
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2025
In this video, we demonstrate the separation of bacterial PCR-amplified DNA using agarose gel electrophoresis. Agarose gel functions as a molecular sieve, enabling the negatively-charged DNA to migrate based upon their size under an applied electric field.
FAQs
Overlapping sequences provide the sequence-specific connection between adjacent DNA fragments. During initial amplification, primers place matching ends on the separate products; those ends can then pair, allowing one fragment to guide extension across the junction of the other. The resulting continuous product can be amplified in later PCR cycles, preserving the designed arrangement in a single DNA molecule.
Primer design determines which fragments can join and where each junction will occur. A primer must amplify its assigned fragment while adding the sequence needed to match a neighboring fragment. Because the overlap is encoded in the primers, Fusion PCR can connect selected regions in a planned order. This design also permits deliberate sequence changes at junctions or within the assembled product.
Unlike restriction-based assembly, Fusion PCR does not require compatible restriction sites at fragment boundaries. This distinction is useful when existing sequences lack suitable sites or when adding such sites would alter the construct. The joining information is instead carried by primer-added overlaps, giving researchers a way to assemble DNA while retaining the intended boundary sequences.
A targeted mutation can be incorporated through primer design so that an amplified fragment carries the intended sequence change before the fragments are joined. The same strategy supports gene fusions or coordinated assembly of regulatory and coding regions. Consequently, the final recombinant product can be used to examine how a selected sequence alteration or arrangement affects gene structure and function.
A typical workflow begins by selecting the DNA regions to be combined and designing primers that add complementary overlaps. Each fragment is amplified separately, after which the products are combined so their overlapping ends can anneal. Extension creates the joined template, and subsequent amplification enriches the full-length recombinant product. The assembled DNA can then support cloning or functional studies.
Researchers use this approach when an experiment requires a defined combination of genetic elements rather than an isolated fragment. It can assemble coding sequences with regulatory regions, create gene fusions, or produce variants carrying targeted changes. These products support genetic engineering and synthetic biology, while also enabling studies of gene organization and function.