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Analysis of SNARE-mediated Membrane Fusion Using an Enzymatic Cell Fusion Assay
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Analysis of SNARE-mediated Membrane Fusion Using an Enzymatic Cell Fusion Assay

Analysis of SNARE-mediated Membrane Fusion Using an Enzymatic Cell Fusion Assay

DOI:
10.3791/4378-v

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09:19 min

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October 19, 2012

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, , ,
1Department of Biochemistry and Molecular Biology,University of Louisville School of Medicine

Chapters

  • 00:05Title
  • 01:19Cell Surface SNARE Expression Analysis
  • 03:29Enzymatic Cell Fusion Assay
  • 06:55SNARE-mediated Interactions between SNAP-25/Syntaxin1/Syntaxin4 and VAMPs
  • 08:46Conclusion

Summary

Automatic Translation

Automatic Translation

We have developed a cell fusion assay that quantifies SNARE-mediated membrane fusion events by activated expression of β-galactosidase.

Tags

SNARE-mediated Membrane FusionEnzymatic Cell Fusion AssaySNARE ProteinsV-SNAREsT-SNAREsIntracellular Vesicle FusionReconstitution AssaysCell Fusion AssayFlipped SNARE ProteinsMicroscopic AnalysisQuantification Of Cell Fusion Eventsβ-galactosidaseTet-Off Gene Expression SystemTetracycline-controlled Transactivator (tTA)Reporter PlasmidLacZ GeneTetracycline-response Element (TRE-LacZ)COS-7 Cells

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