Genetic Rescue
Genetic Rescue - Related Videos
04:46
A Novel Rescue Technique for Difficult Intubation and Difficult Ventilation
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2011
We describe a technique to maintain oxygenation and ventilation using an endotracheal tube inserted nasally to the level of the naso-pharynx while sealing the mouth and nares for successful positive pressure ventilation.
04:38
A Novel Technique of Rescuing Capsulorhexis Radial Tear-out using a Cystotome
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Cited by 4 •
2011
Capsulorhexis is an important step in phacoemulsification surgery. A surgeon creates a continous curvilinear tear on the anterior lens capsule by controlling the tearing vector forces. A peripherally extended tear is a serious complication. This video demonstrates a novel technique of rescuing capsular radial tear out using a cystotome.
12:22
A Parasite Rescue and Transformation Assay for Antileishmanial Screening Against Intracellular Leishmania donovani Amastigotes in THP1 Human Acute Monocytic Leukemia Cell Line
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Cited by 118 •
2012
A parasite-rescue and transformation assay with THP1 cells infected in vitro with Leishmania donovani has been optimized for anti-leishmanial drug screening. The assay involves differentiation of THP1 cells, infection with promastigotes, treatment with test drugs, controlled lysis of the infected macrophages, rescue of amastigotes, transformation to promastigotes and monitoring promastigote growth and proliferation with a fluorometric assay.
03:06
Rescue of Infectious Virions from Cells Transfected with Bacterial Artificial Chromosome-Derived Synthetic RNA
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2026
Source: Yun, S. et al. Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses. J. Vis. Exp. (2015)This video demonstrates the recovery of infectious Japanese encephalitis virus from synthetic RNA transcribed from a bacterial artificial chromosome clone. It shows how cells are electroporated with the synthetic RNA, allowing the viral genome to direct the production of new virions. The resulting plaques formed in infected cell monolayers confirm...
12:34
Adenofection: A Method for Studying the Role of Molecular Chaperones in Cellular Morphodynamics by Depletion-Rescue Experiments
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Cited by 7 •
2016
We describe a method for depletion-rescue experiments that preserves cellular integrity and protein homeostasis. Adenofection enables functional analyses of proteins within biological processes that rely on finely tuned actin-based dynamics, such as mitotic cell division and myogenesis, at the single-cell level.
FAQs
Additional alleles can increase heterozygosity, meaning individuals carry more genetic variation at relevant gene locations. In a small population, this can lessen the effects associated with inbreeding depression and may improve survival or reproduction. The expected benefit is therefore genetic and demographic: healthier individuals can contribute more effectively to population persistence.
The same gene flow that adds useful variation can also produce outbreeding or reduce local adaptation. Outbreeding refers to potential problems arising when genetically different populations are crossed, while local adaptation reflects traits suited to particular conditions. Conservation decisions must therefore balance genetic benefits against the possibility that introduced variation could reduce fitness.
Adding alleles can broaden the genetic variation available to a vulnerable population. That variation may help the population retain evolutionary potential, meaning capacity for future adaptation, rather than remaining limited by a small or isolated gene pool. This makes the strategy relevant not only to current decline, but also to changing environmental conditions.
Biologists must consider whether the proposed source population can provide beneficial gene flow without creating unacceptable risks of outbreeding or loss of local adaptation. The overview identifies genetically appropriate individuals as central to implementation, so the choice is not simply a matter of adding any available animals. It requires matching conservation goals with genetic suitability.
Implementation can occur through managed translocation, breeding programs, or release of genetically appropriate individuals. Managed translocation moves selected individuals between populations, whereas breeding programs incorporate the intended genetic contribution before animals are released or used in recovery efforts. These approaches provide different operational routes for increasing gene flow while keeping the intervention under conservation management.
Genetic rescue is particularly relevant when a population is small, isolated, or experiencing decline and its genetic health has become a conservation concern. Biologists may use it to strengthen vulnerable populations, support recovery, or maintain evolutionary potential under environmental change. Its application connects population genetics with practical decisions about long-term persistence and recovery.
The intended outcomes include greater genetic diversity and heterozygosity, reduced inbreeding depression, and improved survival or reproduction. At the population level, these changes may strengthen a vulnerable group and support recovery from decline. Because benefits can coexist with outbreeding or reduced local adaptation, outcomes must be interpreted as a balance of gains and risks rather than as uniformly positive.