Gfp Tagged Gamma Tubulin
Gfp Tagged Gamma Tubulin - Related Videos
10:41
Using a GFP-tagged TMEM184A Construct for Confirmation of Heparin Receptor Identity
0 Views •
Cited by 2 •
2017
A construct encoding TMEM184A with a GFP tag at the carboxy-terminus designed for eukaryotic expression, was employed in assays designed to confirm the identification of TMEM184A as a heparin receptor in vascular cells.
09:14
Imaging Calcium Responses in GFP-tagged Neurons of Hypothalamic Mouse Brain Slices
0 Views •
Cited by 5 •
2012
In this protocol, we update recent progress in imaging Ca2+ signals of GFP-tagged neurons in brain tissue slices using a red fluorescent Ca2+ indicator dye.
05:00
Monitoring the Arrival of GFP-Tagged Surface Proteins to the Trans-Golgi Network Using Functionalized Nanobodies
0 Views •
2025
This video demonstrates a method to study the uptake and retrograde transport of functionalized nanobodies to understand their intracellular trafficking pathways. By introducing radiolabelled sulfate and utilizing isolation and detection techniques, this method enables the study of endocytic uptake and trans-Golgi network arrival of cargo proteins.
03:44
Generation of GFP-Tagged Recombinant Respiratory Syncytial Virus Using a T7 Expression System
0 Views •
2026
Source: Bouillier, C. et. al., Amplification, and Titration of Recombinant Respiratory Syncytial Viruses. J. Vis. Exp. (2019)This video demonstrates the generation of a green fluorescent protein (GFP)-expressing recombinant respiratory syncytial virus using a T7-based reverse genetics system. The co-transfection of plasmids encoding viral proteins and a GFP-tagged genome leads to protein expression and initiates virus rescue.
03:12
Visualizing Pathogen-Induced Oxidative Stress in C. elegans Using GFP-Tagged SKN-1
0 Views •
2026
Source: Naji, A., et al. Studying Oxidative Stress Caused by the Mitis Group Streptococci in Caenorhabditis elegans. J. Vis. Exp. (2019)This video demonstrates the use of green fluorescent protein-tagged SKN-1 in C. elegans to monitor oxidative stress by tracking green fluorescence. Worms exposed to S. gordonii show strong nuclear fluorescence, confirming pathogen-induced stress, while those fed E. coli display diffuse green fluorescence in the cytoplasm, indicating minimal SKN-1 activation.
FAQs
The gamma-tubulin portion contributes to microtubule nucleation through the gamma-tubulin ring complex, while the GFP portion provides a fluorescent signal for detection. This combination lets researchers relate the position of gamma-tubulin to sites where microtubules begin forming, linking molecular localization with organization of the microtubule network.
These locations concentrate gamma-tubulin because they function as microtubule-organizing centers. Fluorescent enrichment at centrosomes or spindle poles therefore helps reveal where nucleation capacity is positioned within a cell. Comparing these signals can support analysis of centrosome formation and spindle organization during cell division.
Changes in fluorescence location or distribution can indicate how gamma-tubulin organization changes as cells progress through the cell cycle. Because gamma-tubulin is associated with centrosomes and spindle poles, the reporter can help follow formation and organization of these structures and identify altered patterns linked to cell-division processes.
GFP-tagged gamma-tubulin reports the distribution of a microtubule-nucleating component rather than simply marking the entire microtubule network. Its signal is especially informative at microtubule-organizing centers, where gamma-tubulin supports nucleation through the gamma-tubulin ring complex. This distinction helps connect network organization with the sites that initiate microtubule formation.
The reporter can be detected by fluorescence microscopy in either living or fixed cells. Live-cell analysis supports observation of gamma-tubulin-associated structures as cellular organization changes, whereas fixed-cell analysis allows researchers to examine preserved distributions at selected stages. In both settings, fluorescence patterns can be compared with centrosome and spindle organization.
Applications include studying centrosome formation, spindle organization, cell division, developmental processes, and defects in microtubule organization. By showing where gamma-tubulin is concentrated and how that pattern changes, the reporter provides a way to examine relationships between microtubule-nucleating sites and broader cellular or developmental outcomes.