Glass Pipette Needle
Glass Pipette Needle - Related Videos
05:30
Making Patch-pipettes and Sharp Electrodes with a Programmable Puller
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Cited by 22 •
2008
This video shows how to use a programmable puller to make patch pipettes and sharp electrodes for electrophysiology. The same procedure can be used to make a variety of glass tools, including injection needles.
06:35
Introduction to Serological Pipettes and Pipettors
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2023
The serological pipette is frequently used in the laboratory for transferring milliliter volumes of liquid, from less than 1 ml to up to 50 ml. The pipettes can be sterile, plastic, and disposable or sterilizable, glass and reusable. Both kinds of pipettes use a pipet-aid, for the aspiration and dispensation of liquids. Different sizes of pipettes can be used with the same pipet-aid for a variety of experimental assays. For example, serological pipettes are useful for mixing chemical solutions...
01:28
The Looking Glass Self
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2025
The concept of the looking-glass self describes how an individual's self-concept is shaped by their perception of how others see them. This psychological theory, first introduced by sociologist Charles Horton Cooley in 1902, posits that self-identity emerges in a social context and is influenced by the judgments—real or imagined—of others.Research suggests that individuals frequently overestimate how positively others perceive them. This is particularly evident in physical self-perception,...
10:54
In Vivo Three-Dimensional Two-Photon Microscopy to Study Conducted Vascular Responses by Local ATP Ejection Using a Glass Micro-Pipette
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Cited by 10 •
2019
We present an optimized local ejection procedure using a glass micro-pipette and a fast two-photon hyperstack imaging method, which allows precise measurement of capillary diameter changes and investigation of its regulation in three dimensions.
09:51
Intraosseous Needle Placement
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2023
Source: Julianna Jung, MD, FACEP, Associate Professor of Emergency Medicine, The Johns Hopkins University School of Medicine, Maryland, USA For unstable patients requiring urgent administration of medications, fluids, or blood products, establishing vascular access quickly is essential. However, there are many factors that can complicate placement of a peripheral intravenous cannula (PIV), and it is extremely common for PIV attempts to fail. PIV placement may be technically challenging in small...
FAQs
The heating-and-pulling step determines how sharply the capillary narrows and therefore how the working end behaves. A finely tapered tip can deliver or remove very small amounts of fluid and can be positioned for contact with an individual cell. Adjusting the pull produces a needle suited to the scale and purpose of a particular microscale experiment.
Tip diameter and shape are central control variables because they determine how the tool approaches a target cell. A smaller or differently formed tip can be selected when the task requires more localized delivery, aspiration, or membrane penetration, whereas the target cell and intended manipulation guide the adjustment. This tailoring helps researchers conduct controlled cellular experiments.
Glass contributes two practical properties: a rigid shaft that supports precise positioning and chemical compatibility with many biological solutions. Together, these features allow the same general tool design to function in fluid handling and direct cell work. The material is therefore relevant when experiments require both mechanical control and contact with biological samples or solutions.
During membrane penetration, the tapered end concentrates the interaction at a small area, allowing material to enter a cell or enabling intracellular access for a measurement. The narrow geometry supports controlled contact rather than broad disturbance of the cell surface. This principle underlies applications such as microinjection and electrophysiological recording.
A microscope-based workflow begins by matching the needle’s tip diameter and shape to the target cell and task. The needle is then positioned for fluid delivery, aspiration, penetration, or other manipulation, while the operator maintains controlled contact with the sample. This approach supports reproducible microscale handling without treating every cell or experiment identically.
For microinjection, the needle provides a route for introducing material into an individual cell. In biology, that material may include genetic material, making the tool useful for studying cellular function and the consequences of controlled intracellular delivery. The same precision also supports embryo handling, where careful manipulation at small scale is essential.
Glass pipette needles serve different research purposes depending on what must be controlled. Fluid delivery and aspiration address movement of biological solutions, cell manipulation and embryo handling address physical positioning, and electrophysiological recordings address cellular measurements. This range connects the tool to studies of cellular function, genetic material introduction, and controlled microscale biology.