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June 07, 2019
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This protocol is designed to study the slow conducted vascular responses in cerebral capillaries elicited by local ejection of ATP.The advantage of this technique is to simulate local synaptic activities by local pipette ejection and to keep the scant vessel in focus by 3D two-photon microscopy.This method can also be used for studying cerebral neuroma and glial activity at rest and upon stimulation in three dimensions.Demonstrating the procedure will be Michael L
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We present an optimized local ejection procedure using a glass micro-pipette and a fast two-photon hyperstack imaging method, which allows precise measurement of capillary diameter changes and investigation of its regulation in three dimensions.
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Cai, C., Zambach, S. A., Fordsmann, J. C., Lønstrup, M., Thomsen, K. J., Jensen, A. G. K., Lauritzen, M. In Vivo Three-Dimensional Two-Photon Microscopy to Study Conducted Vascular Responses by Local ATP Ejection Using a Glass Micro-Pipette. J. Vis. Exp. (148), e59286, doi:10.3791/59286 (2019).
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