Gridded Glass Coverslip

A gridded glass coverslip is a transparent microscopy coverslip marked with a coordinate pattern that helps researchers locate and document specimens on a slide. During light microscopy, the grid provides a stable reference system, allowing cells, colonies, tissue features, or other microscopic structures to be identified by position and revisited after changing magnification or imaging conditions. In biology, this simple alignment aid supports systematic observation, image correlation, and repeated measurements, improving the consistency of microscopy workflows. It is especially useful when researchers need to track selected regions, compare observations over time, or coordinate imaging with downstream analysis.

Gridded Glass Coverslip - Related Videos

Research

JoVE Journal - Biology

Sequential Application of Glass Coverslips to Assess the Compressive Stiffness of the Mouse Lens: Strain and Morphometric Analyses

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Cited by 28 •

2016

Age-related increases in eye lens stiffness are linked to presbyopia. This protocol describes a simple, cost-effective method for measuring mouse lens stiffness. Mouse lenses, like human lenses, become stiffer with age. This method is precise and can be adapted for lenses from larger animals.

Implantation of Cranial Imaging Window in Mouse Model: A Surgical Procedure to Implant Glass-based Coverslip for Stable Optical Access to Regions of Murine Brain

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2023

This video presents a surgical procedure to implant cranial window in a mouse model for stable optical access to different regions of the brain.

B Cell Activation on an Antigen-Coated Coverslip

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2025

This video demonstrates B cell activation in vitro. The process involves antigen interaction with B cell receptors, leading to cytoskeleton remodeling, immune synapse formation, lysosome movement, antigen internalization, processing, and subsequent presentation on MHC molecules for B cell activation.

Education

JoVE Core - Social Psychology

The Looking Glass Self

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2025

The concept of the looking-glass self describes how an individual's self-concept is shaped by their perception of how others see them. This psychological theory, first introduced by sociologist Charles Horton Cooley in 1902, posits that self-identity emerges in a social context and is influenced by the judgments—real or imagined—of others.Research suggests that individuals frequently overestimate how positively others perceive them. This is particularly evident in physical self-perception,...

Research

JoVE Journal - Bioengineering
Free Sample

Visualizing Proteins and Macromolecular Complexes by Negative Stain EM: from Grid Preparation to Image Acquisition

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Cited by 137 •

2011

Visualizing protein samples by negative stain electron microscopy (EM) has become a popular structural analysis method. It is useful for quantitative structural analysis, such as calculating a 3D reconstruction of the molecules being studied, and also for qualitative examination of the quality of protein preparations. In this article we present detailed protocols for preparing the EM grids, staining the sample and visualizing the sample in an electron microscope. Novice users can follow these...

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