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Sequential Application of Glass Coverslips to Assess the Compressive Stiffness of the Mouse Lens: Strain and Morphometric Analyses
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Sequential Application of Glass Coverslips to Assess the Compressive Stiffness of the Mouse Lens: Strain and Morphometric Analyses

Sequential Application of Glass Coverslips to Assess the Compressive Stiffness of the Mouse Lens: Strain and Morphometric Analyses

DOI:
10.3791/53986-v

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07:56 min

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May 03, 2016

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, , ,
1Department of Cell and Molecular Biology,The Scripps Research Institute

Chapters

  • 00:05Title
  • 01:00Lens Dissection
  • 02:24Stiffness Measurements
  • 04:51Lens Nucleus Measurement and Image Analysis
  • 05:51Results: Axial and Equatorial Strain-load Curves and Morphological Characteristics of 2,4, and 8-month-old WT Mouse Lenses
  • 07:05Conclusion

Summary

Automatic Translation

Automatic Translation

Age-related increases in eye lens stiffness are linked to presbyopia. This protocol describes a simple, cost-effective method for measuring mouse lens stiffness. Mouse lenses, like human lenses, become stiffer with age. This method is precise and can be adapted for lenses from larger animals.

Tags

Keywords: Mouse LensCompressive StiffnessMorphometric AnalysisLens DissectionCoverslip ApplicationBiomechanical PropertiesLens VisualizationEye DissectionLens RemovalCoverslip WeightMeasurement ChamberMicroscopy

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