Hematopoietic Cell Homing
Hematopoietic Cell Homing - Related Videos
10:40
Homing of Hematopoietic Cells to the Bone Marrow
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Cited by 15 •
2009
This article describes a protocol used to study the homing of hematopoietic cells to their niches in the bone marrow.
20:38
Isolation and Transplantation of Hematopoietic Stem Cells (HSCs)
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Cited by 31 •
2007
10:25
Competitive Homing Assays to Study Gut-tropic T Cell Migration
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Cited by 11 •
2011
Competitive homing experiments allow to directly assessing the migratory properties of two different cell populations in a single mouse. Here we illustrate this procedure by comparing the migration of ex vivo-generated gut-tropic versus non-gut tropic T cells.
22:06
Derivation of Hematopoietic Stem Cells from Murine Embryonic Stem Cells
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Cited by 8 •
2007
This protocol details the derivation of transplantable hematopoietic stem cells from mouse embryonic stem cells (ESC) and their subsequent injection into lethally irradiated recipient mice. Briefly, ESC are differentiated as embryoid bodies, which are then infected with retroviral HoxB4 and co-cultured with OP9 stromal cells and hematopoietic cytokines.
14:42
Isolation and Analysis of Hematopoietic Stem Cells from the Placenta
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Cited by 11 •
2008
We have identified the placenta as a major hematopoietic organ during development. We found that hematopoietic stem cells (HSCs) are both generated and expanded in the placenta in unique microenvironmental niches. Here, we describe experimental techniques required for isolation and visualization of HSCs in the mouse placenta.
FAQs
CXCL12 provides a chemotactic signal, meaning it directs cell movement, while CXCR4 serves as the corresponding receptor on hematopoietic cells. Their interaction helps guide blood-forming stem and progenitor cells toward supportive bone marrow niches. This signaling step establishes directional movement before adhesion and tissue entry mechanisms help position the cells within the marrow environment.
Endothelial cells form the vessel lining that hematopoietic cells must cross, while cell-adhesion molecules help the cells attach and move across the vessel wall. Stromal components within the marrow then contribute to local positioning and retention. Together, these interactions connect chemotactic guidance with physical access to, and residence within, supportive tissue niches.
Reaching the marrow is not the only requirement for a useful outcome. Retention within a supportive niche allows hematopoietic stem and progenitor cells to remain in an environment associated with survival, self-renewal, and blood-cell production. Consequently, homing mechanisms must coordinate movement with stable interactions that keep cells in the appropriate microenvironment.
The process can be viewed as a linked sequence: chemotactic signals first direct cells toward marrow, adhesion interactions support contact with endothelial cells, and the cells then cross the vessel wall. Stromal interactions help retain them in the niche. Examining these stages separately helps researchers identify where inefficient movement or positioning may limit later engraftment.
In hematopoietic stem cell transplantation, cells must reach supportive marrow niches after conditioning so that blood formation can be restored. Efficient homing is therefore connected to engraftment, the successful establishment of transplanted cells in the recipient. Studying the signaling and adhesion steps can help explain why delivery to the correct microenvironment matters for recovery.
Homing research supports efforts to improve transplantation, regulate cell mobilization, and develop regenerative therapies. It also provides context for treatments involving hematological disease, where the movement and positioning of blood-forming cells may affect outcomes. Comparing chemotactic, adhesion, and stromal interactions helps researchers focus on distinct stages that could influence therapeutic cell behavior.