Journal
/
Biology
/
Isolation and Transplantation of Hematopoietic Stem Cells (HSCs)
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Isolation and Transplantation of Hematopoietic Stem Cells (HSCs)

Isolation and Transplantation of Hematopoietic Stem Cells (HSCs)

DOI:
10.3791/157-v

•

20:38 min

•

February 25, 2007

•

,
1Center for Regenerative Medicine, Massachusetts General Hospital, Harvard Stem Cell Institute,Harvard Medical School

Chapters

  • 00:00Introduction
  • 00:20Isolating bone marrow cells
  • 00:21Dissecting mouse to isolate bones
  • 01:03Isolating tibia
  • 01:40Isolating femur
  • 02:12Isolating hip bone
  • 02:50Isolating spine
  • 04:07Cleaning bones
  • 06:37Crushing bones
  • 09:33Lineage depletion of bone marrow cells
  • 14:06Antibody staining for FACS
  • 16:06Transplantation into irradiated mouse
  • 18:50Discussion

Tags

IsolationTransplantationHematopoietic Stem CellsHSCs

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Derivation of Hematopoietic Stem Cells from Murine Embryonic Stem Cells

Isolation and Analysis of Hematopoietic Stem Cells from the Placenta

Isolation of Early Hematopoietic Stem Cells from Murine Yolk Sac and AGM

Generation of Human CD40-activated B cells

Isolation and Derivation of Mouse Embryonic Germinal Cells

Lentiviral-mediated Knockdown During Ex Vivo Erythropoiesis of Human Hematopoietic Stem Cells

Phenotypic Analysis and Isolation of Murine Hematopoietic Stem Cells and Lineage-committed Progenitors

Induction and Analysis of Epithelial to Mesenchymal Transition

Isolation, Culture, and Transplantation of Muscle Satellite Cells

Protocol for Isolation of Primary Human Hepatocytes and Corresponding Major Populations of Non-parenchymal Liver Cells

Read Article